﻿254 STRONG. 



Table No. X. — Tests of the agglutinating value of human immune seru — Cont'd. 



Case No. 



Manner of inoc- 

 ulation. 



Dilutions. 



Results and remarks. 







1:5: 1:10: 1:20; 



After 2 hours no precipitate of bacteria in 







1 : 40: 1 : 80: 



any of tubes. After 16 hours marked 







1:160: 1:320: 3 



precipitate in dilutions of 1:5, 1:20, 



VIII 



One agar slant 

 "Pest Aviru- 

 lent." 



controls with- 



j out serum. 



'■ Control normal 



and 1 : 40. 

 After 2 hours moderate precipitation of 





1 human serum, 



bacteria in dilution of 1:10; after 4 







1:5; 1:10; 1:20; 



hours in dilutions of 1 : 5 and 1 : 20. 







3 controls with- 





- 





i out serum. 









1:2; 1:5; 1:10; 



After 2 hours all negative. After 16 hours 







1:20; 5 controls 



marked precipitation with clearing of 







without serum. 



fluid in dilutions of 1 :5, 1 :10 and 1:20. 

 Not in 1 : 2. 



VIII 



do 



Control with 



After 2 hours all negative. After 16 hours 







human serum, 



marked precipitation with clearing of 







1:5; 1:10; 1:20. 



fluid in dilutions of 1:5, 1 : 10, and 1 :20. 







3 controls (no 



Controls negative. 







serum). 









1:2; 1:5; 2 con- 



All negative after 1 hour. After 16 hours 







t r o 1 s (no 



dilution 1:5 marked precipitation. 







serum). 



Other tubes negative. 









Negative after 1 hour. After H hours 

 flocculent precipitate strongly resem- 



IX 



do 



Control normal 



bling agglutination appears in the dilu- 

 tion of 1:10 of one normal serum and in 







human serum 







I, 1:2; 1:10. 



the dilution of 1:5 in the other. Precipi- 







Control normal 



tate disappears on shaking. Dilutions 







j human serum 



of 1 : 2 with each serum negative. After 







1 II, 1:2; 1:5. 



15 hours dilutions of 1 : 10 and 1 : 5, 





- 





heavy precipitate. Dilutions of 1:2 

 1 still negative. 







1:2; 1:5: 2 con- 



All negative after 1 hour. After 16 hours 







trols (no serum). 



moderate precipitate of bacteria in dilu- 



X 



do 



Control normal 



tion of 1 : 5. 







human serum. 



Negative after 1 hour. 







1:1: 1:5. 









1:2; 1:5; 2 con- 



Negative after 1 hour. Moderate precipi- 







trols without 



tate of bacteria in dilution of 1:5 after 



X 



do . 



serum. 

 Control normal 



16 hours. 

 Negative after 1 hour. Moderate precipi- 









human serum, 



tation of bacteria in dilution of 1 : 5 after 







1:2; 1:5. 



16 hours. 



XI 



do 



do— 



Exactly the same results obtained as in 









Case X. 



XII 



do 



1:2; 2 controls 

 without serum. 



Negative after 1A hours. 



XIV 



_ __ do 



1:2: 1:5; 2 con- 

 trols without 



Negative after 1 hour. 









serum. 





