﻿274 



STRONG. 



even a considerable immunity against this disease in the individual in 

 question, since in animals and human beings known to be thoroughly 

 immune to pest infection it has also not been possible to demonstrate 

 these protective substances. 



EXPERIMENTS IN RATS DEMONSTRATING THE ANTI-INFECTIOUS POWER OF 

 PLAGUE IMMUNE SERA. 



Series : 8. — Experiments with the species "Miis decumanus." 



One 48-hour agar slant culture of "Pest Virulent" ( from guinea pig number 

 1221, second transplant) was suspended in 5 cubic centimeters saline solution. 

 A 5 cubic centimeter needle was dipped in the suspension and thrust under the 

 skin near the root of the tail and then withdrawn. Animals numbered 1252, 1254, 

 and 1256 received pest immune serum intravenously just before being stuck with 

 the infected needle. 



Animal 

 No. 



Serum. 



Result. 



Autopsy and remarks. 



1249 





Dead after 4 



No pest bacilli found in smears from heart's 







days. 



blood. Innumerable from region near point 

 of inoculation. Bubonic abscess. 



1250 





Dead after 5i 



Few pest bacilli in heart's blood and in tissues 







days. 



near point of inoculation. Quite numerous 

 in smears from spleen. 



1251 





Dead after 3i 



Numerous pest bacilli in heart's blood. 







days. 





1252 



1.25 cc. antipest 



Alive. _ 







serum intraperito- 









neally. 







1253 





Dead after Si 



Fair numbers of pest bacilli in heart's blood. 







days. 





1254 



1 cc. antipest serum 



Alive __ 



Animal very sick for 7 days after inoculation. 





intraperitoneally. 







1255 





Dead after 3A 



Pest septicaemia. Fair numbers of pest bacilli in 

 heart's blood. 







days. 



1256 



1 cc. antipest serum 



Alive _ _ 







intraperitoneally. 







1257 





Dead after 3 



Smears and cultures from heart and spleen show 







days. 



few pest bacilli. On examining the tissues 

 near the point of inoculation innumerable 

 pest bacilli are found. Animal evidently 

 died of pest intoxication. 



1258 





Dead after 7 



Bubo of the right inguinal gland in which pest 

 bacilli are present. No organisms found in 







days. 









smears from the heart's blood. 



1259 





Dead after 5 



Countless pest bacilli in smears from the tissues 







days. 



near point of inoculation. None seen in 

 smears from the heart's blood. 



1260 





Dead after 2 



No autopsy. 







days. 



