﻿STUDIES IN PLAGUE IMMUNITY. 



277. 



Series 14. — Experiments with rats. 



A 3-day agar culture of "Pest Virulent" (first transplant) was suspended in 

 5 cubic centimeters of bouillon. A 5 cubic centimeter needle was dipped in this 

 suspension and rats numbered 1330 to 1341 inoculated by thrusting the needle 

 subcutaneously near the root of the tail. Rats numbered 1330 to 1337 received 

 intraperitoneal]}', just prior to being stuck with the infected needle, serum from 

 human cases which had previously been vaccinated against plague. 



Animal 



No. 



Serum intra- 

 peritoneally. 



How inocu- 

 lated. 



Result. 



Autopsy and Remarks. 



1330 



4 cc. from 



Stuck with 5 



Dead after 



Fair number of pest bacilli in smears from 





case 11. 



cc. syringe 

 needle. 



3 days. 



spleen. Abdominal cavity appears nor- 

 mal. 



1331 



5 cc. from 

 case 12. 



. do 



do 



Only one pest bacillus found in a smear from 

 the spleen. Countless numbers near the 















point of inoculation. (Edema, hemor- 











rhagic infiltration, etc., about this area. 











Abdominal cavity normal in appearance. 



1332 



5 cc. from 



do ... - 



Dead after 



Very numerous pest bacilli in smears from 





case 9. 





5 days. 



the spleen. 



1333 



do_. 



_ .do 



Dead after 



Innumerable pest bacilli in smears from the 

 spleen. Abdominal cavity normal. 









3 days. 



1334 



2.5 cc. from 



-do 



do 



Innumerable pest bacilli in smears from 

 spleen. 





case 17. 







1335 



do - 



_ __ do 



Dead after 



Abdominal cavity appears normal. No 

 serum present. Fair numbers of pest ba- 









li days. 











cilli in smears made from tissues near 











point of inoculation. 



1336 



2.5 cc. mix- 



do 



Dead after 



Fair number of pest bacilli in smears from 





ture from 





3 days. 



spleen. 





cases 1 8 











and 10. 









1337 



do 



do 



Dead after 



Few bacilli in smears from spleen. Innu- 









4 days. 



merable about point of inoculation. Infil- 

 tration and hsemorrhage about this area. 



1338 





do - 



Dead after 



Fair number of pest bacilli in smears from 

 spleen. 









3 days. 



1339 





do 



Dead after 



Very numerous pest bacilli in smears from 









4 days. 



spleen. 



1340 





do — 





No organisms in smears from spleen. Fair 

 numbers near point of inoculation. 









li days. 



1341 





do 



— _do 



Fair numbers of pest bacilli about point of 

 inoculation. 











