﻿STUDIES IN PLAGUE IMMUNITY. 



279 



Series 19. — Experiments with rats. 



November 0, one 48-hour agar slant culture of "Pest Virulent" (from guinea 

 pig number 1356, first transplant) was suspended in 5 cubic centimeters of 

 bouillon; a 5 cubic centimeter syringe needle was dipped in this suspension and 

 each animal inoculated by thrusting the needle beneath the skin near the root 

 of the tail. Animals numbered 1380 to 1407 received intraperitoneallv, just prior 

 to being stuck with the infected needle, serum from human eases previously vac- 

 cinated against plague. Rat number 1412 received 0.5 cubic centimeters of an 

 antipestic immune serum. 



Animal 

 No. 



Serum intra- 

 peritoneallv. 



How inocu- 

 lated. 



Result. 



Autopsy and remarks. 



1386 



4.25 c c. 



Stuck with 



Dead after 



Very numerous pest bacilli in smears from 





from case 



infected 



3 days. 



spleen. 





19. 



needle. 







1387 

 1388 



do 



do 



do 



Do. 

 Do. 



4 cc. from 

 case 20. 



do. - _ 



. do__. _ 







1389 



do 



_do .. 



Dead after 



Numerous pest bacilli in smears from spleen. 









4 days. 



1390 



2 cc. from 



do 



do 



Numerous pest bacilli in smears from spleen. 





case 21. 







Intestinal wall dark and hemorrhagic in 

 places. 



1391 



3.5 cc. from 



do 



do 



Innumerable pest bacilli in smears from 





case 23. 







spleen. 



1392 



do_ 



.do 



do _ — - 



Intestine hremorrhagic in places. Innu- 

 merable pest bacilli in smears from spleen. 











1393 



3 cc. from 



do 



do 



Innumerable pest bacilli in smears from 





case 24. 







spleen. 



1394 



do 



do 



do 



Very few pest bacilli in smears from the spleen. 

 Innumerable near the point of inoculation. 











1395 



3 cc. from 



do 



__ do 



A few pest bacilli in smears from the spleen. 





case 25. 







1396 



4 cc. from 



do 



do 



Numerous pest bacilli in smears from the 





case 26. 







spleen. 



1397 



. .do 



___ do _ _ 



Dead after 



A very few typical pest bacilli in smears from 









3 days. 



spleen. 



1398 



4.5 cc. from 



do 



do „ . 



A fair number of pest bacilli in smears from 





case 27. 







the spleen, appearing in clumps. 



1399 



.do 



do 





Numerous pest and post-mortem invading 

 bacilli in smears from the spleen. 









4 days. 



1400 



5 cc. from 



do 



Dead after 



No bacilli found in smears from the spleen or 





case 28. 





8 days. 



near point of inoculation. 



