﻿STUDIES IN PLAGUE IMMUNITY. 



281 



Series 20. — Experiments ivith rats. 



November 11, one 70-hour agar slant culture of "Pest Virulent" (from guinea 

 pig number 1.354, first transplant), was suspended in 5 cubic centimeters of 

 bouillon; a 5 cubic centimeter syringe needle was dipped in this suspension and 

 thrust beneath the skin near the root of the tail of each animal. Animals num- 

 bered 1423, 1425 and 1427, 1429 and 1431 and 1433 received intraperitoneally 1 to 

 2 cubic centimeters of an antipest immune serum just prior to being stuck with 

 the infected needle. 



Animal 

 No. 



Amount of 

 serum intra- 

 peritoneally. 



How 

 inoculated. 



Result. 



Autopsy and remarks. 



1423 



2 cc. antipest 



Stuck with 



Dead after 



Carbolic acid poisoning. 





serum. 



5 cc. syr- 

 inge nee- 

 dle. 



half hour. 





1414 





do 



Dead after 



Few bacilli in smears from spleen. Innu- 









4 days. 



merable near point of inoculation. 



1425 



2 cc. antipest 



do . . 



Dead after 



No organisms found in smears near point 





serum. 





11 days. 



of inoculation. In cultures from spleen 

 no pest bacilli. 



1426 





do 



Dead after 



Innumerable pest bacilli in smears from 









4 days. 



tissues near point of inoculation. 



1427 



2 cc. antipest 

 serum. 



do 



Alive 





1428 





_ _do 



Dead after 

 4 days. 



Innumerable pest bacilli in smears from 

 spleen. 







1429 



2 cc. antipest 

 serum. 



do 



Dead after 

 5 hour. 



Carbolic acid poisoning 



1430 





.do 





Very numerous pest bacilli in smears from 

 spleen. 









5 days. 



1431 



1 cc. antipest 

 serum. 



do 



Alive 





1432 





do _ . 





Numerous pest bacilli in smears from the 

 spleen. 









3 days. 



1433 



0.5 cc. anti- 

 pest serum. 



do 



Dead after 

 1 day. 



Probably carbolic poisoning. 



1434 





.... do 



Dead after 

 3 days. 



Numerous pest bacilli in smears from the 

 spleen. 







Series 22. — Experiments with rats. 



On November 17, one 48-hour agar slant culture of "Pest Virulent" (from 

 guinea pig number 1383, second transplant) was suspended in 5 cubic centimeters 

 bouillon, a 5 cubic centimeter needle was dipped in this suspension and thrust 

 beneath the skin near the root of the tail of each animal. Rats numbered 1453 to 

 1463 inclusive received intraperitoneally, just prior to being stuck with the 

 infected needle, serum of monkeys which had been previously inoculated and 

 subsequently had been shown to be entirely immune to large doses of the strain 

 "Pest Virulent." 



