﻿STUDIES IN PLAGUE IMMUNITY. 



283 



Series 27. — Experiments with ruts. 



One 48-hour agar slant culture of "Pest Virulent" from guinea pig numbered 

 1668, first transplant), was suspended in 5 cubic centimeters of bouillon; a 5 

 cubic centimeter syringe needle was dipped in this suspension and thrust beneath 

 the skin near the root of the tail of each animal. Rats numbered 1677 to 1686 

 received intraperitoneally pest immune serum just before being stuck with the 

 infected needle. 



Animal 

 No. 



Inoculated 

 intraperitone- 

 ally with — 



How 

 infected. 



Result. 



Autopsy and remarks. 



1677 



1678 

 1679 

 1680 



1681 

 1682 



1683 

 1684 

 1685 

 1686 

 1687 

 1688 



1689 

 1690 

 1691 



1692 

 1C93 

 1694 

 1695 

 1696 



1 cc. pest 

 immune 

 serum. 

 do 



.do 



Stuck with 

 infected 

 needle. 

 do 



. . do' _. _ 



Dead after 



7 days. 



Alive and 

 well. 



Dead after 

 4 days. 



Dead Jan- 

 uary 31, 

 after 12 

 days. 



Alive and 

 well. 



Dead Jan- 

 uary 22, 

 after 3 

 days. 



Dead after 



8 days. 

 Dead after 



4 days. 

 Dead after 



6 days. 

 Dead after 



3 days. 

 Dead after 



6 days. 

 Dead after 



3 days. 



do 



Very numerous pest bacilli and post-mortem 

 organisms in smears from spleen. 



Innumerable pest bacilli in smears from 

 spleen. 

 Do. 



Innumerable typical pest bacilli from point 

 of inoculation. Few if any organisms in 

 smears from the spleen. 



Fair numbers of typical pest bacilli in 

 smears from the spleen. 

 Do. 



Innumerable pest bacilli in smears from 



spleen. 

 Numerous pest bacilli in smears from spleen. 



Very numerous pest bacilli in smears from 



spleen. 

 No organisms found in smears from spleen. 



Innumerable pest bacilli near point of 



inoculation. 

 Innumerable pest bacilli in smears from 



spleen. 

 Fair numbers of typical pest bacilli in 



smears from spleen. 

 Typical pest bacilli from the local lesion. 



Fair numbers of typical organisms in 



smears from the spleen. 

 Numerous typical pest bacilli in smears from 



spleen. 

 Numerous pest bacilli in smears from spleen. 



Very numerous typical pest bacilli in 



smears from the spleen. 

 Innumerable pest bacilli in smears from the 



spleen. 

 Do. 



_ .do 



do 



.do 



do 



0.5 cc. pest 

 immune 

 serum. 



do 



do . . 



...do 



do 



do — . 



do._ ... 



. do 



do 



. do 





.do 





do 





do 





.do 



_. do .. 





.do 



Dead after 

 17 days. 



Dead after 



4 days. 

 Dead after 



3 days. 

 do 





do 





_do 





do 





. do .. _ 



Dead after 



4 days. 

 do 





do 









55670- 



