﻿284 



STRONG. 



Series 45. — Experiments with white rats. 



October 26, one 48-hour agar culture of "Pest Virulent" (from guinea pig 

 number 2583, second transplant) was suspended in 5 cubic centimeters of bouillon. 

 A 5 cubic centimeter syringe needle was dipped in this suspension and thrust 

 beneath the skin of the animal near tlie base of the tail. Ten of the rats received 

 intraperitoneally 1 cubic centimeter of pest immune serum at the time of being 

 stuck with the infected needle, and ten received the same amount of serum twenty- 

 four hours after the infection. Three received, at the time of the infection, 

 human serum from a case which had suffered from bubonic plague and recovered. 

 The blood was collected five days after the symptoms of the disease had subsided 

 and the serum separated and preserved in 0.5 per cent carbolic acid. The remain- 

 ing 15 rats received no serum. 



Animal 

 No. 



Serum intraperi- 

 toneally. 



Infected 

 October 26. 



Result. 



Autopsy and remarks. 



2595 



Oct. 26, 1 cc. pest 



Stuck with 



Dead Oct. 30, 



No pest bacilli in smears from spleen. 





immune serum. 



5 cc. syr- 

 i n g e - 

 needle 

 dipped in 

 suspen-' 

 sion "Pest 

 Virulent." 



after 4 days. 



Innumerable about point of inoc- 

 ulation. 



2596 



Oct. 27, 1 cc. pest 



do 



Dead Oct. 31, 



Very few bacilli in smears from 





immune serum. 





after 5 days. 



spleen. Numerous in tissues near 

 point of inoculation. 



2597 





do 



Dead Oct. 29, 



Innumerable pest bacilli near point 

 of inoculation. 









after 3 days. 



2598 



Oct. 26, 1 cc. pest 



do 



Dead Nov. 3, 



Very few bacilli in smears from 





immune serum. 





after 8 days. 



spleen. Hemorrhagic area at 

 point of inoculation, containing 

 numerous bacilli. 



2599 



Oct. 27, 1 cc. pest 



do 



Dead Oct. 30, 



Fair number of pest bacilli in smears 





immune serum. 





after 4 days. 



from the spleen. 



2600 





___ do 



do 



Very numerous pest bacilli in smears 

 from spleen. 









2601 



Oct. 26, 1 cc. pest 

 immune serum. 



do 



Alive and 

 well. 





2602 



Oct. 27, 1 cc. pest 

 immune serum. 



_ do 



.__ do .. 









2603 





. . do ___ 



Dead Oct. 30, 



Numerous pest bacilli in smears from 

 spleen. 









after 4 days. 



2604 



Oct. 26, 1 cc. pest 

 immune serum. 



do 



Alive and 

 well. 





2605 



Oct. 27, 1 cc. pest 



do 



Dead Oct. 31, 



Fair number of pest bacilli in smears 





immune serum. 





after 5 days. 



from spleen. 



2606 





do 



Dead Oct. 29, 



Do. 









after 3 days. 





2607 



Oct. 26, 1 cc. pest 



do 



Dead Nov. 1, 



Few pest bacilli in smears from 





immune serum. 





after 6 days. 



spleen. More numerous near 

 point of inoculation. 



2608 



Oct. 27, 1 cc. pest 

 immune serum. 



do 



Alive and 

 well. 





2609 

 2610 





. do 



do 





Oct. 26, 1 cc. pest 



do 



do 





immune serum. 









