﻿286 



STRONG. 



Series 53. — Experiments idth white rats. 



November 6, one 3-day culture of "Pest Virulent" from guinea pig number 

 2594, second transplant, was suspended in 5 cubic centimeters bouillon. A 5 

 cubic centimeter syringe needle was dipped in this suspension and each rat 

 inoculated by thrusting the needle beneath the skin near the root of the tail. Ten 

 of the rats were given subcutaneously 1 cubic centimeter of a pest immune serum 

 at the time of the infection with the needle. Ten others were given the same 

 amount of serum twenty-four hours after the infection, and those which remained 

 alive of the third ten (nine) were inoculated with 1 cubic centimeter of the serum 

 forty-eight hours after the infection. Ten others were given subcutaneously, at 

 the same time as the others, 1 cubic centimeter of this pest immune serum, which 

 however had been treated previously with the virulent pest strain as described on 

 page 261. 







Immune 







Animal 

 No. 



Infected 

 November 6. 



serum, 1 

 cc. subcu- 

 taneously 



Result. 



Autopsy and remarks. 







on- 









2768 



Stuck with 

 5 cc. syr- 

 i n g e 

 needle. 



Nov. 



6 



Alive and 

 well. 





2769 



do 



Nov. 



7 



Dead Nov. 13, 

 after 7 days. 



No pest bacilli found in smears from spleen. 

 One or two swollen organisms found after 

 prolonged search in smears from tissues near 

 point of inoculation. 



2770 



do 



Nov. 



8 



Alive and 

 well. 





2771 



do 



Nov. 



6 



do 





2772 



... do 



Nov. 



7 



Dead Nov. 9, 

 after 3 days. 



Fairly numerous pest bacilli in smears from 

 spleen. 



2773 



do — . 



Nov. 



8 



Dead Nov. 11, 



after 5 days. 



No bacilli in smears from spleen. About point 

 of inoculation haemorrhagic area in which 

 numerous pest bacilli are present. 



2774 



. __do .. . 



Nov. 



6 



Dead Nov. 14, 

 after 8 days. 



Smears from spleen show one or two swollen ba- 

 cilli. Smears from point of inoculation show 

 no organisms. No other evidences of pest in- 

 fection. 



2775 



do 



Nov. 



7 



Dead Nov. 11, 

 afterodays. 



Numerous post-mortem bacilli and a few pest 

 bacilli in smears from the spleen. 



2776 



. do 



Nov. 



8 



Dead Nov. 9, 

 after 3 days. 



Very numerous pest bacilli in smears from 

 spleen. 



2777 



do 



Nov. 



6 



Alive and 

 well. 





2778 



___.do 



Nov. 



7 



Dead Nov. 11, 

 afterodays. 



No bacilli in smears from spleen. Very numer- 

 ous fat point of inoculation. 



2779 



... . do 



Nov. 



8 



Dead Nov. 12, 

 after 6 days. 



Fairly numerous pest bacilli about point of in- 

 oculation. None found in smears from spleen. 



2780 



____ do 



Nov. 



6 



Alive and 

 well. 





2781 



do 



Nov. 



7 



____ do ___- - 





2782 



do. . 



Nov. 



8 



Dead Nov. 11, 

 after 5 days. 



Numerous pest bacilli in smears from the 

 spleen. 



