﻿288 



STRONG. 

 Series 53 — Continued. 





! Immune 









Animal 

 No. 



Infected 

 November 6. 



serum 

 after 

 binding 

 of ambo- 

 ceptors. 



Result. 





Autopsy and remarks. 



2810 



Stuck with 



Nov. 6, 



Dead Nov. 10, 



Pest bacilli 



in smears from spleen. 





5 cc. syr- 



1 cc. 



after9days. 









inge nee- 













dle. 











2811 



do 



____do___ 



Dead Nov. 11, 

 after5davs. 



Do. 





2812 

 2813 



do _ _ 



. do ._ 



do 



Do. 





do 



____do„. 



Alive and 









well. 







2814 



do 



do 



Dead Nov. 13, 

 after 7 days. 



Do. 









2815 



__do 



do___ 



Dead Nov. 10, 

 after 4 days. 



Do. 









2816 



do 



._dC — 



Alive and 

 well. 







2817 



do 



.... do — 



do 







2818 



do 



... .do — 



Dead Nov. 11, 

 after 5 days. 



Do. 





2819 



do 



___ _do__ 



Dead Nov. 13, 

 after 7 days. 



Do. 









Series 57. — Experiments with rats. 



February 23, one 48-hour culture of "Pest Virulent" from guinea pig numbered 

 3079, second transplant, was suspended in 5 cubic centimeters bouillon. A 5 cubic 

 centimeter syringe needle was dipped in this suspension and each rat inoculated 

 by thrusting the needle beneath the skin near the root of the tail. Five of the 

 rats were giving subcutaneously varying amounts of serum from a monkey 

 (numbered 1357*) highly immunized against plague by repeated inoculations in 

 increasing doses of the strain "Pest Virulent." The other rats which served as 

 controls were given no serum. 



Animal 

 No. 



Serum intraperi- 

 toneally. 



How infected. 



Result. 



Autopsy and remarks. 



3087 



3088 

 3089 



3090 



3091 



3095 

 3096 

 3097 



3 cc. serum from 

 monkey No. 1357.* 

 do 



Stuck with 5 cc. 

 syringe needle. 

 do _ 



Alived and well 



Dead after 14 days__ 

 Alive and well 



Dead after 6 days... 



Dead after 4 days___ 



do 



Few pest bacilli in point 

 of inoculation. 



Few pest baci 1 li in smears 



from spleen. 

 Numerous pest bacilli in 

 smears from spleen. 

 Do. 

 Do. 

 Do. 



2 cc. of serum from 



monkey No. 1357. 



. do .. 



do 



do _ 



1 cc. serum from 

 monkey No. 1357. 



... .do. 



.do _ 





do 



Dead after 3 days___ 

 Dead after 4 days___ 





do 









*Monkey number 1357 was inoculated as follows: November 2 infected by sticking with a 5 cubic 

 centimeter syringe needle dipped in a suspension of the strain "Pest Virulent". December 2, 

 one-fifth agar slant culture of Pest "Virulent" subcutaneously. December 14, three-fifths of an agar 

 slant, same strain, subcutaneously. January 10, one agar slant "Pest Virulent," subcutaneouslv. 

 February 1. one and one-half agar slant cultures, "Pest Virulent" subcutaneously. Bled February 

 22. Twenty cubic centimeters; serum tested as above. 



