﻿312 STRONG. 



Series 59. — Showing the attenuation of the strain "Pest Avirulent Manila," in guinea 



Animal 

 No. 



Inoculated. 



Result. 



Autopsy. 



1164 



June 28, two oesen strain "Pest Avirulent 



Dead July 



Numerous pest bacilli in 





Manila" subeutaneously. 



3, after 5 

 days. 



smears from spleen. 



1166 



July 10, two oesen strain "Pest Ayirulent 



Dead July 



Do. 





Manila" subeutaneously. 



16, after 6 

 days. 





1450 



Nov. 14, one-half agar slant culture 



Alive and 







"Pest Avirulent Manila" (attenuated 



well. 







5 months) subeutaneously. 







1578 



Dec. 9, one 48-hour agar slant culture 



Dead Dec. 



Few involution forms of 





"Pest Avirulent Manila" (attenuated 6 



18, after 9 



plague bacilli in the heart's 





months) subeutaneously. 



days 



blood. Six colonies develo- 

 ped in one culture and none 

 in the second. 



1655 



Jan. 12, three-fourths 24-hour agar slant 



Alive and 







culture "Pest Avirulent Manila" (at- 



well. 







tenuated 7 months) subeutaneously. 







1671 



Jan. 15, one 48-hour agar slant culture 



Dead Jan. 



Subacute pest. 





"Pest Avirulent Manila" (attenuated 



29, after 







7 months) subeutaneously. 



14 days. 





1751 



Feb. 11, one 30-hour agar slant "Pest 



Alive and 







Avirulent Manila" (attenuated 71 



well. 







months) subeutaneously. 







Series 60. — Showing the attenuation of the strain "Pest Virulent," in guinea pigs. 



Animal 

 No. 



Inoculated. 



Result. 



Autopsy and remarks. 



1497 



Nov. 27, abdomen shaved and rubbed with 



Dead Dec. 2, 



Numerous pest bacilli 





spleen of guinea pig No. 1495, just dead of 



after 5 days. 



in smears from 





plague from inoculation of the strain "Pest 





spleen. 





Virulent." 







1763 



Feb. 16, one-half oese "Pest Virulent" (at- 



Dead Feb. 20, 



Pest. 





tenuated 3 months) subeutaneously. 



after 4 days. 





2068 



June 7, one oese "Pest Virulent" (attenua- 



Dead June 12, 



Do. 





ted 6 months) subeutaneously. 



after 5 days. 





2069 



do _ _ _ 



Dead June 14, 

 after 7 days. 



Do. 





2070 



do . — _ _ _ 



Dead June 12, 

 after 5 days. 



Do. 





2071 



do 



Dead June 11, 

 after 4 days. 



Do. 





2072 



do____ — . . .. .. 



Dead June 14, 



Do. 







after 7 days. 





2194 



July 23, one oese "Pest Virulent" (attenua- 

 ted 8 months) subeutaneously. 



Alive and well— 





2195 

 2196 



do 



_do ____ _ 



Subacute pest. 



___do 



Dead Aug. 4, 







after 12 days. 



2592 



Oct. 25, two oesen "Pest Virulent" (attenua- 

 ted 11 months) subeutaneously. 



Alive and well_ 





2593 



do . . 



do 









