﻿328 



STRONG. 



Table No. XII. — Showing phenomenon of fixation of the complement with the serwm 

 of human beings vaccinated against plague and with horses' pest immune serum. 



[Saline suspension 0.85 per cent, q. s. to 4 cubic centimeters in each test tube.] 



Inacti- 

 vated 

 rabbits' 

 serum 

 hsemo- 

 lytic for 

 goats' 

 corpus- 

 cles. 



5 per 

 cent sus- 

 pension 

 goats' 

 corpus- 

 cles. 



Guinea 



pig's 

 fresh se- 

 rum for 

 comple- 

 ment. 



Horses' 



pest 

 immune 

 serum 

 (inacti- 

 vated) . 



Vaccinated human 

 serum (inactivated). 



Normal 

 horses' 

 serum 

 (inacti- 

 vated). 



Normal 

 human 

 serum 

 (inacti- 

 vated). 



Saline 

 suspen- 

 sion of 

 strain 

 "Pest 

 Viru- 

 lent." 



Hemo- 

 lysis. 



No. II. 



No. III. 



No. IV. 



0. 0016 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 

 .0033 



1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 



0.15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 















+ 



+ 

 + 

 + 



+ 



-r 



-r 

 

 

 

 

 

 

 



+ 



+ 



-r 

 + 

 ~r 

 -f 

 -r 





 

 

 

 

 

 

 

 

 

 





























1 



0.02 

 .01 

























0.1 















0.1 

 .05 





















.15 







0.05 

 .033 









.15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 

 .15 















.02 

 .01 











1 

 1 



1 













.1 













.1 

 .05 























.05 

 .033 























0.05 

 .01 



0.1 

 .05 





































.05 

 .01 























i 

















.02 











1 

 1 

 1 



1 

 1 

 1 

 1 

 1 





.01 















.1 















.1 

 .05 



























.05 

 .033 































.15 















.0033 



















































Therefore, following plague vaccination in human beings, an immunity 

 reaction occurs which consists in the development of anti -bodies thrown 

 off into the circulation and whose presence may be demonstrated both by 

 the opsonic reaction of Wright and also by the method of the fixation of 

 the complement of Bordet and Gengou. 



Since my first report upon the method of vaccination against plague 

 in man in 1905, such inoculations have been made from time to time 



