﻿REPORT ON CHOLERA IN MANILA. 439 



be interesting to ascertain whether better results could be noted in the 

 treatment of cholera by the use of the antitoxic serum prepared accord- 

 ing to the method recommended by MacFadyan, since he has, according 

 to published reports, prepared the serum of the highest endotoxic power. 41 

 As yet we have not by our own experiments, been convinced of the 

 production in cultures of a soluble toxin by the cholera spirillum, even 

 when it is freshly isolated from the cholera stool. On the other hand, 

 it does not appear to me definitely to have been determined that the toxin 

 which MacFadyan and others have obtained from the protoplasm of 

 the organism, really is the toxin in the same condition in which it gives 

 rise to all of the symptoms of the disease in man. At all events, it 

 would appear that the cell juices which MacFadyan has isolated probably 

 also, in addition to the pure specific cholera toxin, contain certain other 

 poisonous substances. A more extensive routine study of the cholera 

 vibrios isolated freshly from the cholera stools, as well as of the strains 

 of Bacillus dysenteries encountered in dysentery faeces, in relation to the 

 formation by them of soluble toxins, will be pursued in this laboratory 

 as opportunity offers. 



a In a personal communication from Dr. Denier I am informed that Dr. 

 Besredka has recently succeeded in preparing an antitoxic cholera serum which 

 possesses a much greater value than did the serum with which Dr. Denier per- 

 formed his experiments in the treatment of human cases of this disease during the 

 preceding year. 



