﻿454 ASHBTJRN AND CRAIG. 



In searching for Treponema pertenuis in the lesions in monkeys the same 

 methods of securing specimens of the serum and staining them were used as have 

 been already described in Part I of this report. 



We also endeavored to secure cultures of Treponema pertenuis from the lesions 

 in monkeys, using methods similar to those employed in our endeavors to cultivate 

 the organism from human lesions. 



In considering the experimental production of fraruboesia in these 

 animals we were desirous of investigating many problems intimately 

 connected with the subject, aside from the mere successful result of in- 

 oculation, and while we have attempted to solve some of them, we do not 

 feel justified as yet in expressing an opinion regarding our results in 

 certain directions. This is especially true of our experiments regarding 

 re-infection and the local or general nature of the disease as it is observed 

 in monkeys in general, for our experiments in these directions are too 

 few to be of definite value, although they are suggestive. The following 

 protocols of our inoculations include those already completed and those 

 in which it is too early as yet to predict the result. 



PROTOCOLS OF THE EXPERIMENTS. 



Monkey Ao. 1 (3070). — This monkey was inoculated on February 16. 1907, 

 with serum from a typical yaws papule on a young Filipina girl. A sub- 

 cutaneous pocket inoculation was made in the skin of the abdomen, and some 

 of the serum was rubbed into a scarified area over the left eyebrow. Smears 

 of the serum, prepared at the time the inoculation was made, showed numerous 

 examples of Treponema pertenuis. The inoculation wound healed rapidly 

 and the animal appeared normal until March 4. when a small papule covered 

 with a yellowish crust was noticed at the point of the inoculation upon the 

 abdomen; the crust was removed and smears made from the serum which 

 exuded from the minute granulations. An examination of these smears demon- 

 strated the presence of Treponema pertenuis in large numbers. The period 

 of incubation was about sixteen days. Upon March 8, a small, crusted papule 

 had appeared at the point of inoculation over the left eyebrow and smears 

 of serum from this lesion also showed Treponema pertenuis. Both the lesions 

 enlarged slowly, especially the abdominal one, and healing in the center, ex- 

 tended in a circular manner into the surrounding healthy skin. The lesion 

 upon the head had disappeared by May 14, but the abdominal lesion persisted 

 until May 28. Duration of the disease, eighty-two days. On May 15, this 

 animal, still showing a yaws papilloma upon the abdomen, was inoculated 

 with serum from a chancre which contained numerous Treponema pallidum. 

 A subcutaneous pocket inoculation was made in the skin of the abdomen and 

 in addition some of the serum from the chancre was rubbed into an abrasion 

 upon the inside of the left thigh. No results have followed these inoculations 

 to date, June 30, 1907. 



Monkey No. 2 (3071). — On February 16, 1907, this animal was inocu- 

 lated subeutaneously on the abdomen and through an abrasion over the left 

 eyebrow with serum from a yaws tubercle from the same ease as monkey 

 No. 1 (3070). On March 8, a small, crusted papule was observed at the 

 site of inoculation on the head, which gradually enlarged until it reached the 

 size of a small hazelnut ; a typical, yellowish crust developed, which upon 

 removal disclosed the characteristic, pink, granulating surface of a yaws papil- 



