﻿TREPONEMA PERTENUIS CASTELLANI OF YAWS. 455 



Ionia. Examination of the serum from the lesion demonstrated repeatedly 

 the presence of Treponema pertenuis. The incubation period in this case 

 was twenty days. The lesion had entirely disappeared on May 2, thus making 

 the duration of the disease fifty-six days. On May 16 this animal was re- 

 inoculated with yaws serum through a subcutaneous pocket upon the abdomen 

 and an abrasion over the right eyebrow. No lesion has appeared to date, June 

 30. upon the abdomen, but the inoculation wound over the right eyebrow sup- 

 purated and a deeply excavated ulcer resulted. Repeated examinations of the 

 material from the ulcer have always resulted negatively for Treponema pertenuis. 



Monkey No. 3 (3072). — This animal was inoculated February 20, 1907, with 

 serum from a yaws tubercle on a native woman, the inoculation being made upon 

 the abdomen and over the left eyebrow in the manner described. The serum 

 used contained many examples of Treponema pertenuis. On March 8 after an 

 incubation period of twenty days, a small, crusted papule was noticed at the site 

 of inoculation upon both the abdomen and the head. Both lesions enlarged, 

 became covered with a typical crust, and the examinations, which were made 

 repeatedly, -were always positive for Treponema pertenuis. This monkey did not 

 stand close confinement well, became weaker and weaker, and was chloroformed 

 on March 18, ten days after the appearance of the yaws lesion. At autopsy the 

 viscera appeared normal, but the cervical and inguinal glands were slightly en- 

 larged. The yaws tubercle upon the abdomen measured 1 by 0.75 centimeters, 

 was considerably raised above the surrounding skin and covered with a yellowish 

 crust. The lesion upon the head was 1.5 by 1 centimeter, and was very typical 

 of the yaws papilloma as seen in man. The pathologic material was handed to 

 Dr. H. T. Marshall, pathologist of the Bureau of Science, for examination. 



Monkey No. Jf (3073) was inoculated February 26, 1907, upon the abdomen and 

 over the left eyebrow in the manner described, with serum from a yaws tubercle 

 in a native woman. Upon March 8, twenty days after inoculation, a typical 

 yaws papule developed at the site of vaccination upon the head. This lesion 

 enlarged slightly, became covered with the characteristic crust, and the examina- 

 tion of the serum from the granulation tissue revealed upon the removal of the 

 crust showed the presence, repeatedly, of a few Treponema pertenuis. By April 

 16, the lesion had healed, the duration of the disease being about thirty-nine 

 days. On May 15, this animal was inoculated upon the abdomen and right 

 thigh with serum from, a chancre showing many examples of Treponema pallidum. 

 No results have been obtained from this experiment to date, June 30, 1907. 



Monkey No. 5 (A). — This animal was inoculated April 10, 1907, upon the 

 abdomen and right eyebrow, with serum from a yaws tubercle in a leper woman, 

 the inoculation upon the head being subcutaneous, upon the abdomen by rubbing 

 the serum into a slight abrasion. No lesion appeared upon this monkey until 

 May 29, when a well-developed papule about 1 centimeter in diameter and covered 

 with a thick, yellowish crust was observed. This lesion had evidently existed for 

 several days, so that the incubation period is uncertain, probably between thirty- 

 five and forty-five days. Upon removal of the crust the typical pink, rasp- 

 berry-like growth was well marked and examination of the serum from the lesion 

 demonstrated Treponema pertenuis. In this case the lesion enlarged but slightly 

 and by June 12 had disappeared, the duration of the disease being about two 

 weeks. 



Monkey No. 6 (3109).— This monkey was inoculated on March 18 by the 

 subcutaneous pocket method upon the abdomen and through an abrasion upon 

 the forehead, with blood from the heart of monkey No. 3 (3072), obtained at the 

 time of autopsy. No lesions have developed in this animal to date, June 30, 1907. 



Monkey No. 7 (3110). — Was inoculated March 18, 1907, in the same manner 



