﻿4.1C) ASHBURN AND CRAIG. 



as monkey No. 6 (3109) with splenic juice from monkey No. 3 (3072) obtained 

 at autopsy. No lesions Lave developed in this animal to date, June 30, 1907, but 

 there is marked enlargement of the inguinal lymphatic glands. 



Monkey No. 8 (3111). — Inoculated as above with serum from the yaws lesion 

 upon head of monkey No. 3 (3072) obtained at the time of autopsy, March 18. 

 This animal was in a weakened condition from continued confinement at the time of 

 inoculation and died on April 6, nineteen days after the inoculation. No lesions 

 of yaws had appeared at the time of death and the autopsy did not show anything 

 of interest beyond enlargement of the spleen, liver, kidneys and the lymphatics 

 of the abdomen. 



Monkey No. 9 (B). — This animal was inoculated April 13 in the manner 

 already described with yaws serum from a leper woman, the serum having been 

 kept in a glass capillary tube for three days. No lesions have appeared in this 

 monkey to date, June 30, 1907. 



Monkey No. 10 (C). — Inoculated through a subcutaneous pocket upon the 

 abdomen May 15 and through an abrasion upon the inside of left thigh, with 

 serum from a chancre, showing very numerous examples of Treponema pertenuis. 

 No lesions have appeared to date, June 30, 1907. 



Monkey No. 11 (D). — Inoculated May 15 in the same manner as monkey 

 No. 10 (C) with serum from a chancre showing the presence of Treponema 

 pallidum. No lesions have appeared in this monkey to date, June 30, 1907. 



The two latter animals were used as controls to our inoculation of syphilis in 

 yaws monkeys No. 1 (3070) and No. 4 (3073). 



SUMMARY. 



The protocols given show that, in all, eleven monkeys have been used 

 in our experimental work. Of these, five were inoculated directly with 

 serum from human yaws lesions ; one with serum from a human lesion, 

 the serum having been kept in a glass capillary tube for three days; one 

 with blood from the heart of a monkey that had developed yaws ; one 

 with splenic juice from the same monkey ; one with serum from a yaws 

 papilloma, in a monkey; and two with serum from a primary syphilitic 

 lesion. In addition, one monkey after recovery from yaws was reinocu- 

 lated with human yaws serum, and two after recovery were inoculated 

 with syphilis. As regards results; of the five monkeys inoculated with 

 yaws serum taken immediately from the human lesions all developed 

 typical yaws tubercles; the animal inoculated with serum from a yaws 

 lesion in a monkey died before the period of incubation, as shown by our 

 experiments, had expired ; the monkey reinoculated with yaws after 

 recovery has developed no lesions. Lastly, in not one of the four 

 monkeys inoculated with syphilis have any lesions developed. 



PERIOD OF INCUBATION. 



As will be seen upon referring to the protocols, the period of incuba- 

 tion of yaws in the monkeys we experimented with varied from sixteen 

 to about forty-five days, but it shottld be understood that this is 

 only approximate, as owing to the distance of the location of the animals 

 from us and pressure of work, the animals were not inspected every day 

 and thus the lesions may have existed a short time before they were 



