﻿458 ASHBURN AND CRAIG. 



original lesion and in one of onr animals, Monkey No. 1 (3070), such 

 an extension occurred. However, we do not believe the new lesions so 

 produced to constitute a secondary eruption, but simply to be an invasion 

 of the contiguous healthy tissue by the organism causing the disease; 

 that is, the Treponema. Castellani appears to have secured true second- 

 ary lesions situated at a distance from the original papide in his one 

 successful inoculation, and in this case a general infection might be 

 supposed to exist. 



The evidence obtained from our experiments wordd appear to indicate 

 that experimental frambcesia in the monkey, at least in Oynomolgus 

 philippinensis Geoff., is a purely local infection which readily heals after 

 a period of time varying from a few days to several weeks. As we have 

 stated, a few days after inoculation, the wounds had completely healed, 

 although when the infection was conveyed by means of a subcutaneous 

 pocket a slight thickening about the site of inoculation persisted for a 

 short time, finally disappearing before the appearance of the yaws papule. 



In all of our animals the yaws lesion appeared at the site of inocula- 

 tion and when first diagnosed consisted of a small papixle, very slightly 

 elevated above the surrounding skin and covered with a yellowish cap 

 or crust. The papules varied in size from that of a large pin's head to 

 a small pea. The epidermis had been replaced by the yellowish crust, 

 which upon removal revealed a moist surface composed of minute, closely 

 aggregated, but separate, pinkish points, from which a thin, slightly 

 milky fluid exuded. 



The initial papule gradually enlarged, became in most instances eleva- 

 ted, and the crust, formed of the exuded serum, became thicker and more 

 noticeable. The lesions were circular in form and firm iipon pressure. 

 Even when fully developed they were not greatly elevated, as is so fre- 

 quently the case in hiunan yaws tubercles, and in only one of our animals 

 did they project markedly above the surrounding surface. While the 

 crust covering them was always more or less elevated, it would almost 

 invariably be found upon its removal that the granulating surface was 

 but slightly raised, although very distinctly demarcated from the healthy 

 skin surrounding it. 



The crust covering the fully developed yaws lesion in the monkey was 

 perfectly characteristic of that over similar lesions in man, varying in 

 thickness, easily removed, and yellowish-brown in color, sometimes 

 streaked with reddish-brown due to admixtures with blood. 



The surface of the fully developed yaws papule in the monkey, after 

 the removal of the crust, was typical of that observed in human lesions. 

 The color varied from a light pink to a bright red, and a colorless or 

 slightly whitish serum oozed from the raw surface which consisted of 

 minute, closely aggregated papilla?, situated upon a slightly raised base 

 and surrounded by apparently healthy skin. In some of our animals 

 the typical "raspberry" ajmearance, so characteristic of the human yaws 



