﻿ETIOLOGY OF MYCETOMA. 485 



Lactose-litmus agar stab. — The growth on this medium is similar to that 

 on the glueose-agar stab and it produces no change in the reaction of the medium. 



Potato infusion. — The growth is similar to that on bouillon; however, no 

 pigment is produced and the medium is not altered. 



Relation of growth to temperature and resistance to heat. — Growth 

 was observed both at room temperature and at 37° C, but under the 

 former condition it was much slower and less pigment is produced than 

 at the temperature of the incubator. No growth occurs in cultures 

 after exposure to 70° for 15 minutes. 



ANIMAL EXPERIMENTS. 



The series includes forty monkeys (Cynomolgus philippinensis 

 Geoff.), guinea pigs, rabbits, dogs and pigeons. In nearly every in- 

 stance lesions characteristic of mycetoma were artificially produced by 

 intraperitoneal inoculation, with the exception of the rabbits and pigeons. 

 Three typical examples of Madura foot developed in monkeys after 

 injection of the organism into the foot. (See PI. I, figs. 2 and 3.) On 

 the other hand, in no instance was a progressive disease produced by 

 subcutaneous inoculation in other parts of the body. 



INTRAPERITONEAL INOCULATIONS OF MONKEYS. 



Monkey number 3268. — Inoculated into the abdominal cavity with material 

 from the original case. After five days, a small tumor was located above the 

 bladder in the left inguinal region and three days later the animal was found 

 to be in a dying condition. It was immediately killed and autopsy performed. 



Autopsy. — Heart and lungs appear normal ; no fluid in either cavity. Spleen, 

 liver and kidneys normal, some fluid found in the abdominal cavity. A tumor 

 2 centimeters in diameter is present just above and to the left of the bladder. 

 This tumor is surrounded by adhesions and is adherent to the omentum, abdominal 

 wall and intestine, it is extremely firm in the center, with a surrounding 

 (Edematous coat. The cut section shows several small sinuses, from which small 

 quantities of pus may be expressed. Small, distinct, refractive granules can 

 be seen macroscopically, none larger than a pin head. Smears show the pres- 

 ence of a Streptothrix and 8. freeri was reclaimed in pure cultures. 



Monkey number 3269. — Inoculated with the material from the previous animal. 

 After six days the animal showed a disposition to sit in a cramped position. 

 On palpation a nodule, about 2 centimeters in diameter, which seemed to be 

 attached to the abdominal wall was located in the abdominal cavity. Eight 

 days later the animal was found to be in a dying condition. It was killed and 

 autopsy performed immediately. 



Autopsy. — Lungs, heart, liver and kidneys normal. Some fluid is found in the 

 abdominal cavity. Above the bladder, and occupying the entire abdominal cavity 

 there is a firm mass composed of necrotic tissue and compact adhesions of the 

 intestinal coils to the abdominal wall and to the omentum. A cut section shows 

 numerous sinuses surrounded by thickened walls, in some instances these sinuses 

 have burrowed through the walls of the intestines and in others into the wall of 

 the abdomen. A thick, cream-like discharge in which small, glistening grains 

 or granules are visible, can be pressed from the cavities. Pure cultures of the 

 Streptothrix were obtained. 



