﻿486 MUSGRAVE AND CLEGG. 



Monkey number 3307. — Inoculated with 0.2 of a potato slant culture obtained 

 from monkey number 3268. After ten days a small nodule could be felt above 

 the bladder in the left inguinal region. Several days later, multiple nodules 

 developed, and ten days afterward the animal died. 



Autopsy. — Several distinct nodules, which contain thick, cream-like pus are 

 present. Anterior to. and a little above the bladder there is a tumor 2 centi- 

 meters in diameter, bound by adhesions to the mesentery of the descending colon, 

 to coils of the small intestine and to the abdominal wall. On section of the 

 tumor, a heavy, cream-like pus is discharged from the center; the surrounding 

 mass is composed of a necrotic material containing small sinuses filled with pus. 

 Smears show numerous growths of the Streptothrix. 



Monkey number 3308. — Inoculated with 0.25 of a potato slant culture. The 

 animal died ten days later, the lesions being similar to those found in animal 

 number 3307. 



Monkey number 3309. — Inoculated with 0.25 of a potato slant culture in the 

 abdominal cavity. The animal is still living, thirty days after the inoculation. 

 On palpation, a small nodule can be distinguished in the lower portion of the 

 abdominal cavity. 



INTRAPERITONEAL INOCULATION OF GUINEA PIGS. 



Guinea pig number 3313. — Inoculated with a small amount of the culture grown 

 from monkey number 3269. The animal died seven days after the inoculation. 



Autopsy. — Ten cubic centimeters of a clear fluid are found in the abdominal 

 cavity, the heart is slightly dilated, the lungs congested, the diaphragm shows 

 numerous, small, superficial abscesses varying in size, the largest not more than 

 2 millimeters in diameter. The liver is intensely congested and contains similar 

 abscesses, and they are also found in the omentum, mesentery and spleen. Pure 

 cultures of the microorganism were obtained from the abscesses. 



Guinea pig number 3314- — Inoculated with a small amount of the culture grown 

 from monkey number 3269. The animal died ten days later. 



Autopsy demonstrates lesions identical in character with those found in guinea 

 pig number 3313. 



Guinea pig number 3323. — Inoculated with 0.5 cubic centimeter of an emulsion 

 of material from abscesses from monkey number 3269. At the present time, one 

 month later, the animal is still living and seems to be in good health. 



INTRAPERITONEAL INOCULATIONS OF DOGS, RABBITS AND PIGEONS. 



Three healthy dogs weighing about 8 kilograms each were used in these experi- 

 ments. In every ease lesions identical in appearance with those described in the 

 monkeys developed. 



Ten rabbits and three pigeons were inoculated with fresh material from lesions 

 and with cultures of varying ages, but in all cases the results were negative. 



SUBCUTANEOUS AND INTRAVENOUS INOCULATIONS. 



Subcutaneous inoculations into a large number of rabbits, guinea pigs, dogs 

 and monkeys all gave the same results. Small nodules developed only at the 

 points of inoculation; these soon underwent resolution and finally healed. In 

 many instances pure cultures of the Streptothrix were obtained from the 

 discharges. 



Cultures and emulsions of the fresh material were used for intravenous inocula- 

 tion of rabbits, but all the results were negative. 



