﻿494 



MUSGKAVE AND CLEGG. 



of the white variety; it exhibited a condition similar to that cultivated by Vincent, 

 but no ramifications and spores could be discovered. 



"Legrain could not cultivate colonies in infusions of hay, but succeeded in 

 doing so on gelatin with malt and peptone. The colonies were round, prominent, 

 glazed, very adherent to the lower surface, at first pale, then golden-yellow, and 

 soon after became of a vivid red color and finally became decolorized. 



"In the nodules which exhibited suppurative degeneration, Vincent found 

 Staphylococcus pyogenes alius and aureus in addition to the parasites described." 



Vincent gives the following comparative table of the cultural char- 

 acteristics of S. madurce and Actinomyces: 



Table No. I. — Comparison between S. madurae and Actinomyces. 



No. 



Cultures or inoculations. 



Streptothrix madurae. 



Actinomyces. 



1 



2 



3 

 4 



5 



6 



7 



8 



9 



10 



11 



Peptonized beef tea 



Sterilized infusion of hay 



or straw. 

 Ordinary peptone-gelatin __ 

 Gelatin with infusion of 



hay. 



Moderate growth. . 



Luxuriant growth. 

 No growth. 



Liquefies. 



Whitish, very weak 

 culture. 



At first white, later on 

 grayish spots; folded. 



Close, wart-like yellow and 

 white, black-edged col- 

 onies; potato becomes 

 brown. 



No growth. 



Growth. 

 Do. 

 Facultatively anaerobic. 



Transferable to rabbits, 

 guinea pigs, calves. 



Principal nutritive soil: growth 



rapid (4 days) and luxuriant. 

 Does not liquefv 

 Very quick growth; the culture 



becomes pink or red on the 



surface. 

 Colonies which at first are white, 



then pink or red, and umbil- 



icated. 

 Bright pink, vivid or dark red 



culture; does not brown the 



substratum. 



Growth. . 



Potato _ 



Cabbage, yellow turnip. 



carrot. 

 Serum. _ .. 



No growth 



Egg .. 



do 



Cultures in areas deprived 



of air. 

 Inoculations 



do .. . 



Not transferable to any animal — 



8. madurce according to Vincent differs culturally from the fungi 

 isolated by. Nocard, 15 Almquist, 16 and Eppinger. 17 The Nocard organism 

 develops rapidly in peptone-bouillon and it does not grow in hay infusion. 

 The cultural characteristics upon agar and potato present no analog}'. 

 XoearcPs organism is inoculable in guinea pigs. 



Vincent also discusses the question of the identity of actinomycosis 

 and mycetoma and notes the following differences: Potassium iodide 

 does not give the same positive results with Madura foot as it does with 

 actinonxycosis. Different results are obtained with the two parasites, 

 not only morphologically but also in cultures in various media and in 

 inoculation experiments. 



16 Ann, d. I' inst. Pasteur (1888), 2, 293. 



"Ztschr. f. Eyg. u. Infectionskrankh., Leipz. (1890), 7, 189. 



l7 Wien. Klin. Wchnsch. (1890). 



