﻿; II.— Cultural characteristics of 



types of Streptothriw and of ictinomyecs. 



Streptothrix 01' Noenrd accord Id g to the Pullerton. 



Cattle in Guadalupe... 



Staining characteristics . 



Growth on gelatin, 22° C. 



Cultures from t> days to 3 months old stained by 

 Ziebl-Noolson method showed many ;i--iil - 1:1 st 

 portions and these were also alcohol-fast. 



Aerobic. Streak cultures : Growth appears after 

 4 days as a line, white opacity with uueven 

 surface, Slut) cultures: Slightly raised knob al 

 top ol gelatin and a fine, II] my growth alone 

 needle trark. No 1 iquefai 1 ion or pigmentation 

 of medium. 



Appears after 4S hours as a pale, straw-colored 

 growth, with dull, flattened, granular, surface; 

 no pigmentation of medium. 



Growth appears after 24 hours as a grayish, floc- 

 culent mnss at the bottom of the tube. No 



pigmentation of medium. 



Growth occur si. but read ion and appearance of 

 medium not obviously altered after 20 days' 

 Incubation. 



Appears alter IS hours as a b 

 growth. No pigmentation 



■yellow granular 



Potato infusion.. 



y slow growth. No tlquefncl 



Glycerin agar . 



Dinslatfo action... 



Resistance to heat, drying, relative 

 growth to temperature, thermal 

 death point. 



Pathogenicity for lower i 



Joue manifested during 14 days' incubation in 

 starch, peptone and broth. 



■ porulai ing .ulturos resist an exposure to 45° C. 



fur :: inutcs, but are killed at an exposure 



to GO" ('. for the same period. I ('allures resisl 

 exposure in (;-,'■ fur IT. minutes but are killed 

 by exposure to 70° C. for 10 minutes. — 

 (Nocard.) 

 Rabbits, dugs. eats, horses, and oxen are not af- 

 fected by intraperitoneal or intravenous injec- 

 tions. In guinea pies intraperitoneal or intra- 

 v.-ni.ns injections can.-.' . on:- lantl.v within 1M 

 days a milinrv luber. ulnsix similar to that pro- 

 duced by bacillus tuberculosis. 



Streptothrix of Epplnger according In Fullorton. 



Man ; brain abscess . 



:s from 2 to 3 months old stained by 

 -Neelsnti method showed portions which 

 icid-fast but not alcohol-fast. 



Aerobic. Streak cultures: Growth appears after 

 li days as a slightly raised, wrinkled layer with 

 a deep orange center, and a paler oiten iliiuiied 

 out periphery. No liquefaction or pigmenta- 

 tion of medium. 



Growth appears after the fifth day as a slightly 

 raised yellowish mass with a pale. Mat. polished 

 periphery; later the growth assumes a deeper 

 yellow to a barnt-ocher color. No pigmentation 

 of medium. 



Growth appears alter 24 hours as a small, white 

 granules, forming a pellicle mid adhering to 

 sides of tube ; some portions fall 1o the bottom. 

 No pigmentation of medium. 



After 3 days the medium turns pink; by the 

 eighth day the color is discharged and milk has 

 become alkaline, but even alter a month's In- 

 cubation the medium does not clear. 



Irowth appears after IS hours a.- a enmular lay- 

 er, at first while, becoming yellow lo hrlek- 

 red. This Is later covered by a line, white, 

 powdery efflorescence, eultu 

 sugar almond, 



None manifested during 15 days' incubation In 



starch, and peptone broth. 

 Sporulal inc. cultures resist an exposure to G0° C. 



for 30 minutes, but are killed at an exposure 



to 7G° C. for the same period. 



ulc for white mice. Ton 



Aerobic. Grow 



Growth appears after S days as small, discrete 

 yellowish- ah it e colonics with waxy, wrinkle 

 surface; later often developing pink pigment. 



Growth appears as opaque, white, globular, more 

 or less cohering colonies at bottom of tube. 

 Surface growth rare. No pigmentation of me- 

 dium. 



Growth occurs. No obvious change happens in 

 the milk until after 20 days' incubation; the 

 medium then becomes pink and after 4 weeks 

 begins to clear. 



li'nwlli appears after 72 hours as a heuped-up. ir- 

 regular mass of a iTcaiuy-yellnwlsh-whilo color, 

 often showing pah-hes of pink 

 pigmentation or erosion of medium. 



None manifested during 14 days' incubation In 



starch, peptone ami broth. 

 Sporulal inc. cultures resist an exposure to 45" C. 



tor ;:n ininutcs, Inn ,nv killed al an exposure In 



CO" C. for the same period. 



■ Inoculation of > 



Streptothrix of 



Wright according to 



Wright. 







Long b ranch I 







8 u it 



diameter. 



In 



young form 



: ibllea 



e transverse 





re present. 







there i 



ay be swell 





lese points 



.ml 











of oval-ended, 



plump segi 











all. 













be 



seen. The 









lation of la 





al outgrow 



were obser\ 



Gram's stain 





lids. No sp 



ire-boa 



ring organ! 



jms 



m,i We 



serfs fibrin 



stain, 



r modiflcnt 



ons 



could the 



methods. 



Aerobic 



Lin longest. In i 



d Liferent i, illy st a tried by these 



Actinomyces according to Wright. 



All strains of the micro-organism stain well by Gram's 



method with tl xceptlon "t one. Smear preparation 



from cultures of ten cases were not found acid -fast 

 toward Gabliet's decolorizing solut lou after staining 



Anaerobic prefers] 



irowth occurs In bouillon In the form of 

 masses at the bottom of the tube; there It nover 



nodular, Irregular, spherical etna tun , .iit-n ■>■!.■ i cm 



Under continued cultivation most of the strains of the 

 microorganism grow In the form of Oaky, friable. 

 amorphous 'masses, which to some instances after 

 some days In the incubator became transformed Into 

 stringy, viscid material. One strain after months of 

 ■ 1 1 1 r i , it ion i II retained it- mulberry like 



Streptothrix freer! accordinc to Musgr.ive and Clegg. 





i re ad lug, coherent 



n this the growth proceeds as In bouillon except that, no 

 surface layr Is formed. A peculiarity noted in nl.l 

 cultures is the appearance of numerous, black granules 

 about 1 millimeter in diameter in the midst of the 

 mycelium, 



The "rowth appears as a mesh work of widely spreading 

 filaments of a grayish color on the surface of the 

 medium. In old cultures black sclorotia are round as 

 In potato Infusion. In stub .cultures growth appears 

 only at the surface. 



Fourteen strains were planted :l "d ail except two were 

 observed under both anaernbl. and aerobic conditions; 

 ce was anything like a luxurieut growth 



lultlires of five strains were placed under 

 dltions. In no Instance was evidence of growth ob- 

 tained except in one tube. In which there might have 

 been a slight growth. 



,ong.brauehlng filaments, 2 to 7 u In diameter. Trans- 

 verse segments are shown of various lengths from 

 coccold lo 10 u in length. Branching occurs as 

 lateral hypha dc. p.plim from lie •" t: nieltts. The fil- 

 ament3 have a definite wall. Spores have not been 

 observed. 



Smear preparation from lesions mid cultures from r> 

 days" to 2 months' old stained by Zlehl-Neelson's 

 method showed many acid-fast portions. These were 

 also alcohol-fast. Stain well by Gram's method. 



Ibllgatlve aerohic. Growth appears after 3 days as 

 dry, opaque, discrete colonies. No liquefaction. Aft- 

 er 3 months medium becomes slightly darkened. 

 Stab cultures : No growth along needle tract. 



Appears after 3 days as a dry, smooth, opaque and 

 slightly raised growth ; at first a porcelain white, 

 which later develops a delicate pink color. No pig- 

 Growth occurs utter 3 days us flat particles on the 

 delicate, pink color, and on shaking tie tube adhen 



< Irowth appears after 3 days on surfai e of medium as 

 flat, dry-appearing panicles. These later became 

 continent, forming a hoaped-up. pinkish-yellow mass. 



No react ini -in/, in mi ilium until after :■■■■ on. I 



week when the color gradually fades but does not 

 become red. This decolor Issat Ion begins at the bot- 

 tom of the tube. Milk is not coagulated. 



Growth appears ; 's small, pinkish nilnuli -, later rais.d 

 and becoming confluent ; after a few days the growth 

 assumes a Imaped-up appearand resembling a mass 

 of yellow curls. .Medium Incomes moist and dark. 



Growth appears after 3 days smooth and >•( a delicate 

 pink color. Growth does not change character of me- 

 dium .iftcr 3 weeks' incubation. 



Growth appear.: after Z days, later becomes luxuriant 

 with a heaped-up and wrinkled appearance The 

 center assumes rt pink ish-ioilnv, . o|or, with a grad- 

 ual elimination to a delicate pink and to B white 

 ■ripliery. Stab cultures show only surface growth 



Growth observed practically only at 37 C. Resistance 

 to drying showed that the microorganism survived 

 longer on plain surfaces than in culture media, r.n 

 days compared with 6 to 10 days according to strain 

 used. Thermal death point 00° to 64° C. for 10 to 11 



G pigs and rabbits were luuslly used in these Ino- 

 culations. The results were not constant; with 



stains typical bsions win produced which were ana- 

 tomically and histologically Identical with those of 

 actinomycosis. 



with 



anpe irlng. afti r 3 days t 



growth ; produces a : 

 th a pale periphery. 



ied, heaped- 



i exposure to 70" C. for lo mluutes. 



Intraperltoncnl Inoculations of ■-■ 



and dogs i\ lib mat. i nil I < I- -i. 



tures in nearly every ■ . ■ i ■■ I- ■ ■ 

 Ing in deatli after two weeks Rt 

 were not affected other than by the i 

 nodules at site of inoct ' 



pigs, monkeys, 



. in. - 





ploi.!, si 



e produced 



Pure . ullur. 



i experimental I 



