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commencing with August 3 to August 14. I kept track of my morning and 

 evening temperatures * * noting them down in my * * journal 



The afternoon of August 3 I had a chill and a temperature of 38.5°, then around 

 37.2° and 38.3° C. until August 6 when I had another chill and a temperature 

 of 39.2° C, then fever off and on till the 14th of August." 



Examination of blood from the patient. — On June 3 three slides were taken at 

 8 o'clock. Transparent, motile amoeboid bodies moving freely from one red corpus- 

 cle to another were observed in a glass cell. They were evidently parasites which 

 were too debile to enter the corpuscles. Nothing was noted on June 4. On the 

 morning of June 5 the same bodies were seen in the blood serum. They appeared 

 to move with greater vigor. The eosinophiles had attached large numbers of 

 these bodies to themselves and in many instances they had engulfed them. On 

 June 6 a stained slide gave a blue body within the pink corpuscle, but its iden- 

 tification was not certain. On the morning of June 7 the first positive iden- 

 tification of a parasite was made. It was half grown, of the gestivo-autumnal 

 type. It was moving very slowly around an axis without appreciably changing 

 its shape. The nucleus was plainly visible when stained and pigmentation had 

 just begun. Another slide was taken on the same morning and stained by 

 Wright's method. It demonstrated several parasites in the half-grown stage. On 

 June 9 the parasites were again positively identified, also on June 10 to 12, but 

 not on the 13th. On June 14 parasites were observed in the presegmenting stage, 

 and on June 15 and 10 they were seen in all stages up to that of presegmentation. 

 On June 17 a perfect aestivo-autmnmal pre-crescent ring was found, as also on the 

 18th, which was the last day of taking the blood. 



A -noteworthy feature of these blood examinations was the great 

 numbers of parasites that were included by the phagocytes. Parasites 

 which were seen to attack a red corpuscle at one moment would be 

 engulfed the next by an eosinophile. although the phenomenon of the 

 parasite entering the red corpuscle was frequently observed. 



It should be borne in mind that the patient was bitten by infected 

 mosquitoes up to June 6, so that naturally his blood would present an 

 increasing number of parasites in the peripheral circulation toward 

 the latter part of the experiment. 



CONCLUSIONS. 



The work on transmission performed upon the case cited above was 

 in every way of such a positive character, the after effects so marked and 

 the previous history of the patient so well ascertained that there remains 

 no doubt in my mind as to the validity of the tests made. However, it 

 seems very desirable, in order to subject this question to further concise 

 test, that a number of volunteers be secured to undergo, in properly con- 

 structed cages, an isolation period followed by inoculation by Myzomyia 

 ludloivii Theob., and as soon as the season of abundance for these mosqui- 

 toes returns I shall carry out further experiments looking to this end. 

 The results of the continuous experiment at Olongapo have been published 

 at this time as it may not be possible to furnish further proof until several 

 months have elapsed, and it was not thought desirable to delay the 

 publication of the work so far done. 



