120 Miss Willcock and Mr Hardy, Preliminary Note , etc. 
second case, we found the ash to give no test for phosphorus, 
a result which agrees with Hopkins’ analysis of the ash of 
carefully washed albumin. In the former case, in which we 
added bases, abundant phosphorus was present in the ash. This 
would seem to show that the phosphorus is “organic.” 
Estimations. 
I. Neumann’s Method. Proteid prepared by acetic acid method. 
2nd crystallisation OT28°/ 0 P 
4th „ 0127 
6th „ 0-126. 
II. Neumann’s Method. Proteid prepared by acetic acid method. 
2nd crystallisation OT26°/ 0 . 
III. Neumann’s Method. Proteid prepared by sulphuric acid 
method. 
2nd crystallisation 0T81 °/ 0 . 
IV. Pemberton’s Method. Proteid prepared by acetic acid 
method. 
2nd crystallisation OT4O°/ 0 * 
V. Woy’s Method. Proteid prepared by sulphuric acid method. 
2nd crystallisation 0T40°/ 0 - 
The mean value therefore is OT8°/ 0 . This agrees fairly well 
with Osborne and Campbell’s value of OT24°/ 0 . This constancy 
throughout so many fractions furnishes good reason for regarding 
the phosphorus as being a part of the molecule. 
Hopkins found 1 '57 °/ 0 of sulphur present. If the phosphorus 
be taken at our figure OT8°/ 0 it points to there being in the 
molecule 12 S to IP and the molecular weight of the proteid 
works out at 28800. 
