366 
Messrs Purvis and Wilks, The influence 
N 
21 c.c. of— NaHO were required to neutralise the excess after 
Kjeldahling 10 c.c. of the wort. 
The wort was pitched on May 6, and the average temperatures 
between May 7 at 10 a.m. and May 11 at 9 a.m. were: 
dark = 2 3 ‘8° C. 
white = 24-0 „ 
blue = 23-9 „ 
The fermentation in the red was not continued owing to an 
accident. 
The following numbers give the results of the analysis of the 
various fermentations : 
(1) Optical activity for 100 % solutions at 15° C. 
dark = + 2 '10° 
white = + 2 ‘37 
blue = + 2*31 
(2) Copper oxide reducing power in 20 c.c. of 50 °/ 0 solutions: 
dark = -0725 gm. Cu. 
white =-0970 „ 
blue = -0660 
(3) Specific gravity : 
(4) 
dark = 997*88 
white = 998*23 
blue = 997 '94 
The nitrogen content ; 10 c.c. of each fermentation was 
... N 
Kjeldahled, and the NH 3 distilled into 25 c.c. of acid. The 
N 
following numbers represent the amount of — alkali used to 
neutralise excess of acid : 
dai’k = 22*95 c.c. 
white = 23*60 „ 
blue = 23*37 „ 
(5) The acidity. The following numbers represent the amounts 
N 
of — alkali required to neutralise 50 c.c. of each of the various 
fermentations : 
dark = 7*77 c.c. 
white =12*47 ,, 
blue = 6*20 „ 
The small development of acidity in the dark and blue is very 
marked. 
