462 Mr Brindley, Regeneration in Samia ailanthus. 



The results obtained under (B), (C) and (D) above differ from 

 those of Newport and Chapman in two respects, viz. the terminal 

 claw apparatus was as a rule not present in the regenerated limb, as 

 it was characteristically in their cases, and in Samia there was no 

 case in which the tarsus was regenerated with the normal number 

 of joints, while Chapman has figured at least three cases of a 

 5 -jointed tarsus in Liparis. It is quite possible that generic 

 differences exist in these respects. The experiments on Samia 

 tend to suggest that the earlier the instar injured the more the 

 imaginal limb approaches the normal in form and size. As 

 regards the comparative results of injuring a leg to a greater or 

 lesser extent at the same larval stage, the results obtained under 

 (G) and (D), which it was hoped would throw light on the point, 

 were attended with so much variability, and were, owing to the 

 numerous deaths in pupa, so few in total number, that all that 

 can be said is that there is at least a suggestion that an injury 

 at the onset of pupation is, if confined to the middle joint of the 

 leg, followed by the production of a rather better formed imaginal 

 leg than if it includes removal by amputation through the basal 

 joint. So far as it goes, there is here some evidence in favour 

 of Gonin's conclusion 1 , based on anatomical examination, that just 

 before pupation only the extremity of the developing pupal leg 

 projects into that of the larva, and that amputation of the latter 

 at its base is therefore likely to injure the pupal leg more exten- 

 sively than section through the middle joint. Chapman's observ- 

 ations on Liparis were undertaken in part with the object of 

 examining the evidence for Gonin's view, and he arrives at a con- 

 clusion unfavourable to this author, but I am unable to agree with 

 the interpretation he places on his results with Liparis in this 

 respect, though, as stated above, my own experiments on Samia 

 are too few to do more than suggest that Gonin's view is the 

 correct one. 



1 Bull. d. 1. Soc. Vaudoise d. Sci. Natur., 1894, s^r. 3, xxx., p. 122. 



