SHORT NOTES. 317 



of another kind (conidia) are budded off: such cases of different 

 kinds of reproductive bodies are well known to all mycologists. 



The small globular spores have been made to. germinate, but 

 died at an early age in all cases from the attacks of introduced 

 organisms (Bacterid, Torulat), &c, which are so troublesome in 



these experiments. 



The question what becomes of the long and curled tubes emitted 

 by the papillate spores has been followed up with more success 

 lately. The following facts have been obtained by growing the 

 spores on the under side of thin glass squares, so arranged as to 

 form the roof of a small cell kept moist by wet blotting-paper; 

 various contrivances to avoid the introduction of foreign fungi need 



not here be detailed. 



Having attained a considerable length, all the colouring- 

 matter, &c, passes along a branch, and a pear-shaped swelling is 

 formed at its end ; this receives all the contents, a septum forms 

 for the first time, and wo have a swollen reddish body separated oft 

 from the empty tube and spore, which shortly die off. 



This coloured body has been seen to send out branches. A 

 number of rather large hyaline motile bodies (zoospores) generally 

 make their appearance, and two of these have been seen to fuse. 

 The connection between these various facts demands further 



enqU f y ' ****** 



Numerous attempts to directly infect coffee-plants with "leaf- 

 disease" have as yet failed. I may select the following irom a 

 multitude of examples as to methods adopted :— 



1. Papillate spores were sown on the under side ot a young 



leaf, covered with a glass cell, and placed m various 

 positions, temperatures, and dry or moist atmospheres 



2. Similar sowings were made on thin slices o coftee hat kept 



moist in glass cells ; some in water, others in various 



solutions. ' , . , ■ 



8. Sections of -disease-spots" were treated as above to try 

 and induce the mycelium to spread from the leaf-passages, 

 either on to other slices or on to the coftee-leaf . 

 4. Several of the spores found on the coffee-leaf have been 

 sown as above! The chief cause of failure here has been 

 the unavoidable introduction of other forms which increase 

 so rapidly as to destroy the selected ones before their 

 normal fate has become indicated. 



SHORT NOTES. 



POTYMOaMON TBICHOIOES, CluUH., IN HvST SUFFOLK. - On 



Au»u ILl 1 gathered PoUnno.eton trickles at -f™^"* 



Earl Norfolk, guided by directions kindly given me by Lev. hirby 

 Trimmer its 'discoverer' in England. It occurred but sparingly. 



