The Philippine Journal of Science 



mi 



Experiment. 



6. Ten grams of faeces were added to 40 cubic 



centimeters of 0.4 per cent solution of caus- 

 tic soda. 



7. Ten grams of faeces were added to 40 cubic 



centimeters of 0.2 per cent acetic acid. 



8. Ten grams of faeces were added to 40 grams 



of 1 per cent mercuric chloride. 



9. Ten grams of faeces were added to 40 grams 



NaCl (0.8 per cent) cooled to 5° C. 



Result after 6 hours. 

 Balantidia living and 

 motile. 



Balantidia dead. 



Result after 5 minutes. 



Balantidia dead. 

 Balantidia dead. 



Ortman (1891) observed the action of certain chemicals on 

 balantidia in hanging-drop preparations as follows : 



Chemical. 



Strength of 

 solution. 



Time of 

 exposure. 



Result. 



NaCl 



} per cent 



H. mi. 

 45 



Balantidia dead. 

 Negative. 

 Balantidia dead. 



Do. 



Do. 

 Some of the balantidia livintr. 



Do. 

 Balantidia dead. 



Do. 



Do. 



Do. 





Potassium permang^anate 



1:3,000 

 2:2,000 

 1:3,000 

 1:1,000 

 1: 400 



30 

 20 

 13 

 20 

 25 

 11 

 3 

 2 30 

 5 



Hydrochloric acid. 



Do - 



Acetic acid . _ - 





Do... 



Do .- 



1: 200 

 1: 100 



Quinine sulphate . _. 



1:2,000 

 1:1.000 



Do 





In the experiments described in this paper, an attempt has 

 been made to determine quantitatively the balantidicidal action; 

 first, of those drugs and chemicals which have been more or 

 less successfully employed in the treatment of other protozoan 

 infections, and, if these failed to be active; secondly, to seek 

 for some efficient balantidicidal drug or chemical that could 

 be used in the treatment of balantidial dysentery. The first of 

 these purposes has been limited to some extent by my inability 

 to procure in Manila all of the drugs and chemicals that have 

 been employed in the treatment of protozoan diseases. How- 

 ever, a certain number of them have been obtainable which 

 represent the chief groups of these substances, as the aniline 

 dyes, the arsenic and the antimony compounds, ipecac and its 

 alkaloid, emetine, and the salts of quinine. The second purpose, 

 to seek some new drugs or chemicals having a specific action 

 on Balantidium coli, has also proceeded according to a definite 

 plan which will be apparent later. 



