VIII. B, 1 Boynton: Normal Blood of the Carabao Q\ 



blood, which correspond very closely to those found in cattle. 

 Following the widely used classification they are (1) lymphocyte, 



(2) large mononuclear, (3) polynuclear, (4) eosinophile, and 



(5) mast cell. 



Lymphocytes include cells averaging 7.3 microns in diameter, 

 being a little larger than the red corpuscles, and having a nucleus 

 occupying a greater part of the cell body. The nucleus is usually 

 round, but may show a notch on one side. The cell body shows 

 as a narrow rim around the nucleus. Both nucleus and cell 

 body are coarsely reticular. The cell body has a strong affinity 

 for basic stains, and the nodal points in the reticulum take a 

 deeper stain. With Jenner's and Wright's stains, a few small 

 purplish granules are frequently seen in the cell body. With 

 eosin and methylene blue, both nucleus and cell body take a deep 

 blue stain, often the cell body staining deeper than the nucleus. 

 With Ehrlich's triacid mixture, the nucleus takes a greenish color, 

 the cell body has a purplish tinge, and appears homogeneous. 

 This variety of cell is nongranular, except for the few purplish 

 granules numbering from 1 to 5 frequently seen, as has been 

 previously mentioned. 



Large mononuclear leucocytes include cells which are con- 

 siderably larger than the lymphocytes, averaging 10.8 microns 

 in diameter. The nucleus occupies from one-half to two-thirds 

 of the cell, and is situated at one side of the center. It is either 

 oval or kidney-shaped. Both nucleus and cell body are finely 

 reticular, and stain less deeply than do those of the lymphocytes. 

 Frequently small clear areas or vacuoles are seen in the nucleus, 

 giving it the appearance of undergoing degeneration. With 

 Ehrlich's triacid mixture, the nucleus takes a greenish tinge, and 

 the cell body has a very faint pinkish tint. With eosin and 

 methylene blue, the nucleus is light blue and the cell body is 

 distinctly blue, but neither nucleus nor cell body takes such a deep 

 stain as the lymphocytes. With Jenner's stain, both nucleus and 

 cell body are stained blue, but not so strongly as in the case of 

 the lymphocytes. With Wright's stain, the nucleus is dark violet, 

 and the cell body pale blue. 



Transitional forms of the large mononuclears were noticed 

 frequently. In these cells the nucleus is saddlebag-shaped. 

 The staining properties are similar to those already described 

 for the large mononuclears. 



In polynuclear leucocytes the nucleus takes on various shapes. 

 It may be S-shaped, W-shaped, Z-shaped, coiled or lobulated, and 

 is coarsely reticular. In appearance they are very similar to 

 those in human blood. The cell body remains practically un- 



