62 The Philippine Journal of Science ms 



stained, but contains many fine granules, which are so small that 

 they appear as mere points, and show a rather weak affinity for 

 acid stains. The granules are more numerous than the coarser 

 granules in the eosinophiles. These cells average 9.4 microns in 

 diameter, or nearly twice the diameter of the red corpuscles. 



With Jenner's stain, the nucleus is blue and the granules are 

 a bright pink. With Wright's stain, the nucleus has a dark 

 violet color and the granules are distinctly pink. With Ehlirch's 

 triacid mixture, the nucleus takes a pale greenish color, and the 

 granules a pinkish violet. With eosin and methylene blue the 

 appearance is similar to that described for Jenner's stain. 



In eosinophile leucocytes the nucleus is very similar to that of 

 the polynuclears, usually being bilobed or trilobed. As a rule 

 the nucleus takes the basic stains readily, the lobes being coarsely 

 reticular. The cell body contains many oval granules, which are 

 much coarser than the granules in the polynuclears, and are 

 strongly acidophile. These cells are a little larger than the 

 polynuclears, averaging 10.9 microns in diameter. 



With eosin and methylene blue, the nucleus is stained blue and 

 is coarsely reticular. The granules are a bright pinkish red, 

 taking the eosin stain. With Jenner's and Wright's stains, 

 practically the same appearance is presented as with eosin and 

 methylene blue. With Ehrlich's triacid mixture, the nucleus 

 takes a very faint green appearance, while the granules take a 

 deep copper color. 



In mast cells the nuclei are similar in shape to those of the 

 polynuclear leucocytes and eosinophiles, but the nucleus takes the 

 stain so faintly that it is often difficult to determine the shape. 

 The cell body remains practically unstained, but contains many 

 coarse granules, which have a strong affinity for basic stains. 

 The granules are either spherical or slightly oblong in shape, and 

 are practically the same size as the granules in the eosinophile 

 cells. 



With eosin and methylene blue, both the nucleus and granules 

 are stained blue. The granules take a much deeper stain than 

 the nucleus. With Jenner's stain the granules are deep violet, 

 and with Wright's stain they are deep purple, the nucleus being 

 very faintly stained. With Ehrlich's triacid mixture, the nucleus 

 is stained a very light green, while the granules are stained a 

 blackish green color. 



Table I gives the results of the measurements of 100 cells of 

 each variety except the mast cells in which 26 were measured. 

 These measurements were made from the blood of 10 different 

 animals. 



