136 



The Philippine Journal of Science 



1913 



fuging for about twenty minutes disclosed a moderate number 

 of biscuit-shaped, Gram-negative diplococci, which were chiefly 

 within polynuclear cells. The polynuclears were very numerous ; 

 mononuclears, rare. Cultures on Loefler's blood serum showed 

 a pure growth of a Gram-negative diplococcus on the following 

 day. Subsequently, transplants were made to two tubes of 

 each of the following culture media. 



Solid media. 



1 

 Liquid media. 







Slants. 



Stabs. 





Whole human-blood acid 



Lactose litmus agar 



Peptone + 1. 



ajyar-ag-ar. 



-t- 1.' 



Lactose bouillon. 



Loefler's blood serum. 



Glucose agar. 



Plain bouillon + 



Ag-ar-ag-ar + 1. 





1. 



Agar-agar — 1. 







Glycerine agar-atrar. 







Potato. 







In no case was the growth luxuriant. The best results were 

 obtained with the whole human-blood acid agar-agar, while 

 those on Loefler's blood serum ranked second. The grovii;h on 

 acid and alkaline agar-agar and on glycerine agar-agar was 

 very scanty, only isolated colonies developing. Potato media 

 gave negative results. Of the stab transplants, one of each 

 variety gave a very slight growth along the line of puncture, 

 and one of each resulted negatively. No growth was obtained 

 in fluid media excepting in one peptone tube, and this was 

 unsatisfactory since it was contaminated with staphylococci. 



The isolated colonies were somewhat elevated, yellowish, 

 moist, and rather irregular in outline, regardless of the culture 

 medium used. As they became older, they were white and dry 

 with serrated edges. Smears from the cultures showed the 

 diplococci lying for the greater part in single pairs. Tetrage- 

 noid forms were not infrequent; clumps were present; but 

 chains were absent. 



The organisms behaved variously toward Loefler's methylene 

 blue. Those from fresh cultures took the stain well as a rule, 

 whereas those from a 48-hour culture took it poorly. Many of 

 them were swollen in these smears, thus losing some of their 

 biscuit shape. It was necessary to make transplants not less 

 than once in forty-eight hours, in order to keep the culture alive. 



Fermentation tests gave positive results with glucose and 

 maltose; negative results with saccharose, lactose, and mannite. 



