140 



The Philippine Journal of Science 



191S 



Table I shows the results obtained. Horse 1 tolerated 50 

 standard test doses of tetanus toxin six weeks after having re- 

 ceived the immunizing dose of serum ; horse 2 tolerated 50 doses 

 of test toxin two weeks after having received the serum; and 

 the control horse 3 died on the seventh day after having been 

 inoculated with toxin. 



EXPERIMENT NO. 1 A 



Horse 4 received 1,500 units of antitetanic serum on June 

 5, 1912. Horse 5 was inoculated with 1,500 units of antitetanic 

 serum on July 5, 1912, and 1,500 units were given to horse 6 on 

 August 5, 1912. Horse 7 was used as control. 



On October 5, 1912, each horse received 50 standard test doses 

 of tetanus toxin subcutaneously. 



Table I A. 



No. of 

 animal. 



Anti- 

 tetanic 

 serum 

 units. 



Date. 



Tetanus 



toxin 



test 



doses. 



Date. 



Weeks 

 after 

 serum. 



Result. 



Date. 



4 



5 



6....... 



7 



1,500 

 1.500 

 1,500 

 none 



June 5,1912 



do 



Aug. 5,1912 



50 

 50 

 50 

 50 



Oct. 5. 1912 



do 



do-. 



do 



16 

 12 

 8 



Died 



Died . 

 Died- -- 

 Died... 



Oct. 9, 1912 

 Oct. 10,1912 

 Oct. 17,1912 

 Oct. 9, 1912 









As is shown in Table I A, all horses in this series died. 

 Immunity had disappeared eight weeks after the injection of 

 the serum. 



EXPERIMENT NO. 2 



Of 8 guinea pigs, Nos. 1, 2, 3, 4, 5, 6, 7, and 8, each received 

 three subcutaneous injections of antitetanic serum from horse 

 as follows: February 27, 1911, 10 cubic centimeters; March 4, 

 10 cubic centimeters; March 11, 10 cubic centimeters. 



From May 18, 1911, on every fourth day one guinea pig of the 

 series received subcutaneously 250 units of antitetanic serum 

 as follows: Guinea pig 1, May 18; 2, May 22; 3, May 26; 4, May 

 30; 5, June 3; 6, June 7; 7, June 11; and 8, June 15. 



On June 27, 1911, twelve days after guinea pig 8 received the 

 last injection of antitetanic serum and forty days after guinea 

 pig 1 received the last injection of antitetanic serum, each of 



