VIII, B, 2 



Ruediger: Immunity against Tetanus Toxin 



141 



the 8 guinea pigs was inoculated with 0.05 of the test dose of 

 tetanus toxin. Guinea pig 9 served as control ; it had not been 

 treated with serum. 



Table II. 



No. of 

 . animal. 



Anti- 

 tetanic 

 serum. 



units. 



Date. 



Tetanus 

 toxin 

 test 

 dose. 



Date. 



Days 

 after 

 serum. 



Result. 



1 



1- 



2 



3 



4 



5 



6 



7.. 



8 



9. 



250 

 250 

 250 

 250 

 250 

 250 

 250 

 250 

 none 



May 18,1911 

 May 22.1911 

 May 26.1911 

 May 30,1911 

 June 3,1911 

 June 7,1911 

 June 11, 1911 

 June 15, 1911 



0.05 

 0.05 

 0.05 

 0.05 

 0.05 

 0.05 

 0.05 

 0.05 

 0.05 



June 27, 1911. 



do 



do 



do 



do 



do 



do_ 



do 



do . . . 



40 

 36 

 32 

 28 

 24 

 20 

 16 

 12 



Died. 



Died. 1 

 Lived. 1 

 Lived. 1 

 Lived. j 

 Lived. 1 

 Lived. 

 Lived. 

 Died. 









The results, as shown in Table II, were as follows: Guinea 

 pigs 1, 2, and 9 died while 3, 4, 5, 6, 7, and 8 lived. Guinea 

 pig 3 tolerated 0.05 of the test dose of tetanus toxin on the 

 thirty-second day after having received the antitoxin, while 

 guinea pig 2 did not tolerate the same dose of toxin on the thirty- 

 sixth day after injection of antitoxin. 



EXPERIMENT NO. 3 



Guinea pigs 10, 11, 12, 13, 14, 15, 16, and 17 each received 

 three subcutaneous injections of normal horse serum as follows : 

 February 27, 1911, 10 cubic centimeters; March 4, 10 cubic 

 centimeters; March 11, 10 cubic centimeters. 



Beginning on May 18, 1911, every fourth day one guinea pig 

 of the series received an injection of 250 units of antitetanic 

 serum subcutaneously. Injections were given on the following 

 dates: Guinea pig 10, May 18; 11, May 22; 12, May 26; 13, May 

 30; 14, June 3; 15, June 7; 16, June 11; 17, June 15, 1911. 



On June 27, twelve days after guinea pig 17 had received 250 

 units of antitetanic serum and forty days after guinea pig 10 

 was inoculated with antitoxin, each guinea pig in the series was 

 inoculated subcutaneously with 0.05 of the test dose of tetanus 

 toxin. Guinea pig 18 was used as control. 



