VIII, B, 3 Boynton: Note upon Strangles 239 



scopic examination large numbers of cocci and a few short chains contain- 

 ing from 3 to 4 elements each. On the 28th, the agar culture showed 

 several colonies of Micrococcus -pyogenes aureus and albus. Besides these 

 there were a few small grayish colonies which, upon microscopic examina- 

 tion of hanging-drop and stained smear preparations, proved to be strep- 

 tococci. Long chains of streptococci were also found present in the 

 bouillon culture but were mixed with numerous micrococci. Subcultures on 

 agar and in bouillon were made from the small agar colonies. On April 

 29 the subcultures in both media revealed pure cultures of streptococci. 



On May 1, 1 cubic centimeter of bouillon culture was injected subcuta- 

 neously into each of 2 rabbits and 2 guinea pigs, and 5 cubic centimeters 

 subcutaneously into the neck of a horse 1 year of age. The rabbits and 

 guinea pigs suffered no ill effects from the injection. The horse on the 

 second day after injection showed a large swollen area around the point 

 of inoculation which was very tender. On the fifth day the swelling was 

 14 centimeters in diameter. The lymphatic vessels extending from it 

 were swollen, giving an identical picture of a typical, local mallein reaction 

 in a glandered horse. On the eighth day the abscess thus formed broke 

 and discharged a large amount of yellowish blood-stained pus, which 

 upon microscopic examination of stained smear preparation revealed large 

 numbers of streptococci in short chains. 



At the time the abscess broke the horse ate very little and had a 

 depressed appearance, lying down the greater part of the time. In a 

 couple of days it regained its appetite, the swelling gradually subsided, 

 and complete healing took place in fifteen days. The neighboring lym- 

 phatic glands were observed frequently, but no sign of enlargement or 

 tenderness could be detected. 



From all appearances, this inoculated horse had a mild attack of 

 strangles, the infection remaining localized at the point of inoculation. 



Case 3. — On April 25, a 2-year-old horse, which occupied the stall next 

 to that of horse 2, was observed to have a slight discharge from both 

 nostrils. It was immediately isolated, and the premises thoroughly dis- 

 infected. In three days it had a marked discharge from both nostrils, 

 and both submaxillary glands were considerably enlarged. After ten 

 days' duration the discharge ceased, and the glands gradually returned 

 to normal size. A few short chains of streptococci were found on micro- 

 scopic examination of the nasal discharge. In fourteen days this animal 

 had apparently recovered from the attack. 



Case A. — On May 3, a small black mare, 1 year old, whose stall was 

 next to that of horse 3, was observed to have a discharge from the right 

 nostril. The animal was immediately isolated, and the premises thoroughly 

 disinfected. The following day there was obsei-ved a discharge from 

 both nostrils, aiid the submaxillary glands were slightly enlarged. At the 

 end of ten days the discharge from both nostrils had ceased, and the 

 glands were practically normal in size. Recovery was apparently com- 

 plete in fourteen days. 



CONCLUSIONS 



1. From the results derived from the cultures and from 

 microscopic examinations of the purulent discharges, it is evident 

 that streptococcic infection exists in horses in the Philippine 

 Islands. 



