Table I. — Feeding experiments with cultures of Amcebx. 



1 previous to experiment. 



Experi- 

 ment 

 No. 



VII 



vni 



XIV 

 XV 



XVII 



XVIII 



XIX 



ITimeun- 



'derobser- 



' I vation in 



prison. 



1 Yrs. mos 



6 



1 6 



6 



1 6 



5 I 



5 9 



11 - - -iu * *^ -1 * Physical examination of 



History with reference to dysentery. ■' nhdompn 



Dysentery 16 years ago-. 

 Negative 



Dysentery 16 years ago,. 



i attacks of dysentery 6 years ago . 



Neirative 



1 attack of dysentery 8 years ago . 

 Dysentery 16 years ago 



3 attacks of dysentery 6 years ago.. 



Negative 



Dysentery 16 years ago . 



3 attacks of dysentery 6 years ago.. 



Mucous dysentery 7 years ago 



Bloody mucous stools 2 years ago.. 



Dysentery 3 years ago _ 



Negative \ do . 



3 attacks of dysentery 6 years ago ---| do . 



Negative 



Thickened bands along 

 sigmoid. 



3 feeding e^eriments. 



None. _ 

 ....do. 



Culture of Amosba lA 12 days previously with negative 



results. 

 Culture at Ammha SA 12 days previously with negative 



results. 

 Culture of Amffifia 4C 80 days previously with negative 



results. 

 Culture of Amceba SA 136 days previously; culture of 



Amceba 9F121 day s previously ; culture of Amceba lOG 93 



days previously. Results of all three feedings negative. 

 None - --» 



..do- 



Culture of Amceba lA 70 days previously; culture of 

 ArtKeba lOC 34 days previously. Resulta of both feed- 

 ings negative. 



None 



-do. 



Culture of Amceba 2A 36 days previously with negative 

 results; culture of Amceba 2A 24 days previously with 

 negative results. 



Culture of Amceba 8A 9 days previously with negative 

 results. 



None 



Culture of Amceba lA 36 days previously; culture of 

 Amceba lA 24 days previously. Results of both feed- 

 ings negative. 



Culture of Amceba SA 43 days previously; culture of 

 Amxeba 9FM days previously. Results of both feed- 

 ings negative. 



None --- 



-do. 



Culture of Am.ceba ISH IQ days previously with negativ 



results. 

 None 



Motile Entamoeba coli . 

 Negative 



Culture of stools on 



Musgrave and Clegg"; 



medium. 



Source and description of material ingested. 



Source of culture. 



Manila water supply.. 



Clover, U.S.A.... 

 Hay, Illinois, U. S. 



Algas, Kansas, U. S. A_ 

 Normal stool, Manila _.. 



Diarrhceal stool, Kansas, U. S. A-. 



Dysenteric stool, Manila,, 



Strain. ; Species. Stage of development. 



i Encysted 



! Motile and encysted . 

 ' Encysted 



Encysted and motile.. 

 Encysted 



Encysted... 

 Motile and e 

 Encysted ... 



Quantity of material fed. 



Growth on 2 Petri-plate culture 



do 



Growth on 4 Petri-plate cultun 



Growth on 4 agar-slant cultures .. 

 Growth on 7 Petri-plate cultures . 



Growth on 1 agar-slant culture .. 

 Growth on 2 agar-slant cultures , 

 Growth on 4 agar-slant cultures . 



Growth on 2 agar-slant cultures _. 

 Growth on 4 Petri-plate cultures.. 

 Growth on 2 Petri-plate cultures.. 



Growth on 3 agar-slant cultures . 

 Growth on 4 agar-slant cultures . 



Growth 

 Growth 

 Growth 



I 3 Petri-plate cultures .. 

 1 4 Petri-plate cultures., 

 I 4 agrar-slant cultures .. 



I 3 Petri-plate cultures.. 



Material fed with - 



Nothing - 



do_.. 



Magnesiui 



Nothing 



Magnesium oxide.. 



Time under 

 observation 

 after feeding. 



Results of feeding experiments. 



Culture of stools on Musgrave and Clegg's medium. 



Yra. mos. days. 



2 7 



11 7 



2 6 



11 7 



2 4 15 



2 1 15 



Amceba B recovered on first and fifth days after feeding . 



Negative _ 



^7na36a D recovered on first day after feeding 



Amceba D recovered on second and third days after 

 feeding. 



Amrrfta ^recovered on firstand second days after feeding... 



Negative 



Amceba ^recovered on second day after feeding 



Encysted Entamoeba eoli^ 

 Negative 



Ammba F' recovered on first day after feeding do . 



j4.»7«E6a G recovered on firstand second days after feeding.. .1 do . 



Amceba G recovered on second day after feeding ! do . 



ccba G recovered on sixth day after feeding.. 



first to third days after feeding., 

 n first day after feeding 



Amceba G recovered oi 

 Ajiiceba FI recovered < 

 Amceba //recovered on firstand second days after feeding.. 



Am(r6(t ii'recovered on first to third days after feeding 



ft Already parasitized with Entam.ceba coli at the time of feeding Amceba E. 



b This man subsequently became infected with Entamoiba histolytica and developed a slight Attack of dysentery on the 36th day after feeding. 



