288 The Philippine Journal of Science 1913 



Hartmann (1908) states that this species is as a rule less pathogenic 

 for cats than Entatnceba histolytica. Of 3 cats used in his experiments, 

 1 did not develop dysentery, 1 showed after from eight to ten days slightly 

 bloody stools for a few days only, and the third developed a more severe 

 dysentery and died three weeks after infection. 



Werner (1909) experimented with 5 strains of "Entamceha tetragena." 

 Two of these strains when injected into the rectum of cats gave rise to no 

 infection, the other 3 strains produced a dysentery in the experimental 

 animals. One of these latter strains was passed through 5, another through 

 3 cats, and a third through 1 cat, after which their virulence was lost. The 

 period of incubation is given as from five to twelve days with an average of 

 seven and one-half days. The duration of the disease in cats was from eight 

 to thirty-two days. Of the successfully infected cats, 6 died. These showed 

 typical ulcerations of the large intestine, and one had an abscess of the liver. 



Franchini (1911) introduced into the rectum of a healthy monkey, which 

 had been under observation in the laboratory more than one year, fseces 

 from a case of tropical dysentery containing blood, mucus, and numerous 

 "Entamoeba tetragena." Three injections of this material were made, on 

 February 10, 18, and 20, respectively. On May 10 the monkey developed 

 dysentery with numerous entamoebse in his stools. At necropsy the caecum 

 was found to contain one large and numerous small ulcers, and the rest 

 of the intestine showed more or less colitis. "Entamceha tetragena" was 

 found in the intestinal contents and in sections of the large intestine. 



Darling (1912) fed 2 kittens with the cysts of "Entamceha tetragena" 

 from a case of entamoebic dysentery. On the twelfth day both kittens 

 had prolapse of the rectum following intussusception and entamoebic enter- 

 itis. He was unable, in numerous feeding experiments with monkeys, dogs, 

 and cats, to infect with the motile or trophozoite stage of this entamoeba. 



Craig (1913) quotes a personal communication from Dr. H. B. Fantham 

 who had succeeded in producing dysentery in 1 of 2 kittens fed faeces 

 containing "Entamceha tetragena" from an infection contracted in Algeria. 

 The kitten died in three weeks, and ulcerations containing "Entamoeba 

 tetragena" were found in the intestine. All of his experiments by rectal 

 injections of the material into kittens were negative. 



"Entamceha mimita" was found by Elmassian (1909) in the 

 stools of a case of recurrent dysentery in a European who had 

 resided in Paraguay, South America. This Entamoeba had, in 

 the living organism, an indistinct nucleus like Entamoeba his- 

 tolytica, but in stained preparations the nucleus showed a heavy 

 peripheral ring of chromatin like Entamoeba coli. No distinction 

 existed between ectoplasm and entoplasm, and its movements 

 were sluggish. Small cysts, 12 to 14 microns in diameter, were 

 developed which contained 4 nuclei. The author considers this 

 species to be pathogenic, but no experiments were undertaken to 

 prove its pathogenicity. 



In a previous paper (Walker, 1911) I have expressed the 

 opinion, based upon morphological evidence, that "Entamoeba 

 tetragena" Viereck is identical with Entamoeba histolytica Schau- 



