378 The Philippine Journal of Science wis 



and it was much more difficult to inject a substance into them 

 than into healthy cells. 



In order to obtain a more rigid test of the resistance of this 

 plant to serums, the fungus was planted in a soft medium consist- 

 ing of agar one-half and the strongly agglutinating serum one- 

 half. The mycelium grew well, and after one or two days' 

 growth was inoculated with cholera vibrios. Within five hours 

 the inoculated filament was swarming with actively motile vib- 

 rios, and the next day the filament and its branches were dead 

 with vibrios penetrating to the finer endings. A portion of the 

 medium removed with a capillary pipette and applied to a hang- 

 ing drop of cholera vibrios caused immediate clumping. It is 

 evident from this experiment that infection with high motility 

 of vibrios goes on unchecked, even when the host is grown in 

 a highly agglutinating medium. 



To summarize, the cellulose wall of the fungus is apparently an 

 effective barrier against the agglutinins of both cholera and 

 Bacillus pyocyaneus serums. 



Somewhat difl'erent results were obtained as regards the per- 

 meability of this plant for acids. Since Achlya will tolerate a 

 degree of acidity in the medium decidedly harmful to cholera 

 vibrios, it was possible to determine if the progress of an infec- 

 tion might not be altered by changes in the reaction of the 

 medium. 



As a protocol a detailed description of one of this series of 

 experiments is given: 



April 4, morning, A small mycelium grown from a resting 

 spore was placed in a hanging drop of the water of condensation 

 of alkaline agar to which a small quantity of nearly neutral 

 glucose broth had been added. 



April 4, 5.30 p. m. Above medium removed and cholera 

 peptone plus about 0.1 volume of glucose broth substituted. 



April 5, 10.15 a. m. (room temperature 28"". 6). Inoculated 

 with a fresh culture of cholera vibrios. 



April 5, 11.00 p. m. Filament infected, with motile vibrios 

 present. Withdrew alkaline medium and substituted an acid 

 medium consisting of distilled water 5 parts and 1 per cent 

 glucose broth, 5 + acid, 3 parts. A previous test had shown that 

 cholera vibrios were unable to grow in a hanging drop of this 

 medium. 



April 6, 9.30 a. m. (temperature 28°.3). A portion of the 

 inoculated filament is dead, but with few vibrios in it. Branches 

 I and 2 are living and contain vibrios with little or no motility. 



