380 The Philippine Journal of Science wis 



host massed into a sphere containing no or very few vibrios, 

 took on a wall, and later grew into the infected part, now dead. 

 It was found more difficult to infect filaments in even a slightly 

 acid medium. 



In summary, Achlya filaments in which cholera infection is 

 not too much advanced may apparently be "cured" by the addi- 

 tion of a weakly acid medium, but the wall and protoplasm of 

 the living plant evidently offers some resistance to the passage 

 of the acid. Vibrios inside were less affected than those with- 

 out, and highly infected filaments were difficult to cure. 



The dysentery bacillus inoculations were made with an old 

 culture of the Shiga-Kruse type. Some four successful infec- 

 tions were accomplished. While the bacilli grew well, in some 

 cases filling the filament for a considerable distance from the 

 point of inoculation, the destruction of the host was much less 

 rapid than in the case of the motile bacteria. In one experiment 

 an infected filament lived nearly, if not quite, five days at high 

 room temperature. During at least three days of this time the 

 bacilli were numerous in the host; but its protoplasm continued 

 to move, though in contact with dense masses of bacteria. In an- 

 other case an Achlya, taken directly from a water culture among 

 algae, was inoculated. A portion of the infected mycelium lived 

 six days after infection. 



Bacilli of bubonic plague also grew well in the living filaments 

 of Achlya. In one experiment a highly virulent culture, the 

 first twenty-four hours' growth from an infected guinea pig, 

 grew well, and formed chains which extended far from the 

 point of inoculation and into neighboring branches. The hypha 

 inoculated and other parts of the mycelium in connection with 

 it died within twenty-four hours. After the death of the host 

 rhizoid-like filaments from neighboring hyphas surrounded and 

 penetrated the infected filament apparently obtaining food from 

 the disorganizing mass of bacilli. A similar attraction of masses 

 of bacteria for Achlya was seen in dysentery infections. This 

 behavior of Achlya does not prove that the bacteria possess no 

 toxins for Achlya, since toxins, like agglutinins, may be unable 

 to pass the cellulose wall. However, in dysentery at least, there 

 is little evidence of the formation by bacteria inside the wall of 

 toxins destructive to the host. 



Some interesting results were obtained by the inoculation of 

 spores of an Aspergillus isolated from moldy bread. A single 

 spore was inoculated into the vacuole of a filament of Achlya 

 growing in pure culture in a hanging drop of glucose agar. In 



