VIII. B, 6 Schobl: Plague in Manila in 1912 425 



The liver was somewhat enlarged. The organ showed prominent struc- 

 ture, the centers of the acini being red, the periphery lighter in color. 



The kidneys were slightly enlarged and pale. The capsule peeled off 

 easily, the venae stellatse were prominent, the surface smooth; there were no 

 hasmorrhages. The cortex was increased in breadth and was of the same 

 color as the surface; the pyramids were darker in color. The organ was 

 of fragile consistence. 



Suprarenals were normal, as were also intestine and bladder. 



The histological findings were as follows: 



Bubo. — The capsule of the gland was cedematous. The whole gland as 

 seen in cross section had undergone necrosis, except a few foci which still 

 showed cellular structure. 



Lungs. — The alveoli were filled with homogeneous masses, containing but 

 few degenerated epithelial cells and leucocytes. The blood vessels were 

 dilated, particularly in the subpleural part of the organ. In some places 

 capillary mycotic emboli with subsequent haemorrhage were encountered. 

 The large blood vessels and bronchi were normal. 



Salivary gland. — Those glands attached to the primary bubo showed the 

 normal structure of a combined mucous and sei'ous gland. 



Liver. — There was considerable congestion. The centers of the acini 

 showed parenchymatous and fatty degeneration. The cells on the periphery 

 of the acini exhibited typical fatty infiltration. The large blood vessels and 

 small ducts were without change. 



Kidney. — The cells of the kidney showed various degrees of degeneration, 

 ranging from parenchymatous to fatty infiltration. There were a few 

 capillary hasmorrhages and hyaline casts present. 



Suprarenals. — These showed slight degeneration. 



Spleen. — This organ showed congestion, a few haemorrhages, and bacterial 

 emboli; otherwise normal. 



The bacteriological examination of the material from this cat 

 gave the following results: 



1. S7nea7-s: 



a. From the buboes showed degenerated leucocytes, many lymphocytes, 

 and numerous bacteria, some of which resembled Bacilhis pestis in 

 their polar staining. 



h. From the spleen showed numerous plague-like, polar-stained bacilli. 

 Round involution forms were present. 



2. Cultures: 



a. From the buboes were badly contaminated with Bacillus coll and 



Bacillus pyocyaneus colonies. 



b. From the spleen: A few scattered colonies of Bacillus pyocyaneus 



developed on the surface of the agar. Between the large colonies 

 a scanty growth of dewy appearance was noticed. Smears made 

 from this growth revealed plague-like bacilli of the cultural type, 

 showing a few club-shaped involution forms. Subcultures were 

 made in order to secure pure culture. They showed a pure growth 

 of Bacillus pestis as indicated by the morphology of bacilli and 

 shape of the colonies. Agglutination with plague-immune serum 

 was positive. 



122778—2 



