VIII, B, 6 Ashburn et al.: Inoculation of Monkeys 431 



on the third day, followed by a drop, and a second rise on the 

 sixth day, with almost continuous elevation to the seventeenth 

 day. The chart (chart 1) is attached. We call attention to the 

 probability of this rise being due to variola sine eruptione, 

 the eruption being absent because of the protection afforded by 

 the vaccination in October. 



Monkey 19. — A rather small unvaccinated male was inoculated 

 at 5 points on the abdomen on December 4 with fresh vesicle 

 contents. The temperature chart (chart 2) is attached, and 

 attention is invited to its resemblance to chart 1. 



On December 8 it was noted that all of the points of scarifi- 

 cation were reddened. On the 10th the areas of redness were 

 more widely extended and marked, and the belly wall about them 

 was deeply indurated. On the 11th dry scabs were forming 

 and the induration was slightly less. On the 12th a papule was 

 noted on the scalp ; on the 13th 5 papules were found on the scalp 

 and legs. By the 15th a fairly profuse eruption of small vesicles 

 and pustules, some of them ruptured, was seen on the face, arms, 

 legs, and about the anus, while the lesions on the abdomen had 

 further subsided, the induration and swelling about them greatly 

 lessened, and the sites of inoculation were marked by dry scabs. 

 On the 17th the redness and induration had almost entirely dis- 

 appeared from the belly, the scabs had fallen from the inserts, 

 and deep holes marked their location. On the 19th the end of 

 the monkey's tail was seen to be much injured, as though crushed 

 or bitten (probably bitten by an old male. No. 5, tied near), and 

 all variola lesions were scabbed and dry. By December 23 the 

 animal was pronounced well. 



This case we considered variola inoculata in the monkey, char- 

 acterized by fever and signs of local inflammation on the fifth day, 

 by primary and secondary eruptions (the latter appearing on the 

 ninth day) , and by continued fever for about seventeen days. 



Monkey 20. — A small unvaccinated male was inoculated at 4 

 points on the belly with fresh vesicle contents on December 4. 

 The temperature chart (chart 3) is attached, and attention, 

 invited to its resemblance to charts 1 and 2. 



Summarizing the above experiments with fresh vesicle con- 

 tents, we may say that inoculation with it, by way of scarifications 

 of the skin: (1) caused variola inoculata in two unvaccinated 

 monkeys, the primary lesions, secondary lesions, and tempera- 

 ture curves being alike in the two instances and probably char- 

 acteristic; (2) caused in a vaccinated monkey a fever very 

 similar to that produced in variola inoculata, but gave rise to 



