vin. B. 6 



Ashburn ct al.: Inoculation of Monkeys 



435 



By the 8th the induration, redness, and swelling were all 

 beginning to diminish. Dry scabs covered the points of inser- 

 tion. No secondary lesions developed. On January 7 some of 

 these scabs were raised and the beds on which they rested 

 scraped. These scrapings and the triturated scabs were used 

 to inoculate monkeys 8 and 16. 



Summarizing the above we may say that vesicle contents, 

 capable when fresh of causing variola inoculata in monkeys, 

 so loses its virulence by being kept for twenty-four days in the 



Chart 5.— Temperature chart of monkey 23. 



ice chest as to be no longer capable of producing the typical 

 disease with prolonged fever and primary and secondary lesions. 



It did produce an ephemeral rise in temperature in both in- 

 stances after an incubation period prolonged beyond the ordinary 

 length, and in one of the two instances it gave rise to abortive 

 and atypical primary lesions. In neither instance did secondary 

 lesions or severe disturbance result. 



The above finding would indicate that a working and satisfac- 

 tory smallpox prophylactic might be secured by storage and 

 attenuation of virulent vesicle contents, but prophylaxis by vacci- 

 nation as practiced is so safe, satisfactory, and efficient that the 

 pursuit of the clue appears at present unnecessary. 



