436 ^'^s Philippine Journal of Science wis 



II. EXPERIMENTS WITH SCABS OR "DISKS" FROM THE ABOVE CASE OF 



SMALLPOX IN MAN 



As the lesions on the person of the Dutch traveler matured 

 and the scabs fell or w^ere picked off, they were all collected 

 and saved ; one-half of them were placed in glycerin and one-half 

 were placed dry in a sterile test tube. 



On December 19, the patient's sixteenth day in the hospital 

 and about the twenty-third day of his sickness, some of each 

 lot of scabs were triturated in saline solution and some with 

 the serum of monkey 6 (a vaccinated monkey), so as to make 

 thick suspensions. With these suspensions monkeys 28, 22, 26, 

 27, and 29 were inoculated, 5 or 6 insertions being made on the 

 belly of each. 



Monkey 28. — This monkey received scabs preserved in glycerin 

 and triturated with vaccinated monkey's serum. No local lesions 

 developed. On the eighth and tenth days the monkey showed 

 sharp rises of temperature, as indicated by chart 6. He there- 

 after appeared well. 



The sites of inoculation were first reddened on December 

 8 ; on the 10th the redness and induration were very marked, as 

 in monkey 19. On the 11th small vesicles or pustules marked 

 the insertions, and two of them were ruptured. The next day 

 the swelling and redness had begun to subside and the lesions 

 were scabbed. On the 13th small secondary lesions, papules, 

 were seen on the legs and about the anus. On December 15 a 

 profuse eruption of small vesicles and pustules, more numerous 

 than in monkey 19, was present on the palms, arms, legs, face, 

 and scalp. The abdominal lesions were subsiding, and the in- 

 flammatory process in the abdominal wall was almost gone. On 

 the 17th the belly wall was more inflamed and indurated and the 

 swollen ridges were black on top ; apparently secondary infection 

 had occurred. All the secondary lesions were either pustules 

 or scabs. On December 18 the tops of the swollen ridges on the 

 belly sloughed, leaving extensive ulcers, and it may here be stated 

 that these ulcers were not completely healed until the end of the 

 month. Numerous pustules of the secondary lesions were yet 

 unscabbed, but by December 21 all had become so, and desquama- 

 tion was completed by the 26th, the completion being delayed on 

 the palms, where the disks were held down by thickened epider- 

 mis, and on the legs, where entanglement of hairs in the scabs 

 doubtless delayed it. 



This case we also regard as one of variola inocidata in the 

 monkey, characterized by fever and signs of local inflammation 



