VIII. B. li Ashbuni et al.: Inoculation of Monkeys 439 



IV. EXPERIMENTS WITH SCABS FROM VARIOLOUS MONKEYS 



While monkeys 19 and 20 were suffering from their variola, 

 attempts were made to obtain vesicle contents from them, but the 

 vesicles were so small and so soon ruptured by the animals that it 

 was found impracticable. Scabs were collected, however, as the 

 lesions dried, and these were used to inoculate monkeys 24, 25, 

 30, and 31. The results in all of these animals were quite nega- 

 tive, with the exception of monkey 24. 



Monkey 24. — A medium-sized female was inoculated December 

 19 with scabs from monkeys 19 and 20. No general or febrile 

 disturbance resulted, but on December 26 there was swelling, 

 redness, and marked induration of 3 points of insertion and their 

 surroundings. The lesions formed dry scabs. The induration 

 persisted about ten days, and the monkey remained well. 



Whether or not the above monkey suffered from modified 

 primary lesions of smallpox we cannot know positively, but it 

 seems probable. At any rate, all of the above experiments with 

 smallpox scabs or disks from man and monkeys indicate that 

 such material has but feeble virulence and that such as it has is 

 speedily lost. 



On January 7, 1913, the scabs were lifted from the lesions on 

 monkey 23 (see above) , the underlying tissue curetted, and the 

 pulp so obtained used to inoculate monkeys 8 and 16. 



Monkey 8. — A monkey that had been vaccinated in October 

 with the vaccine scab from a pig, atypical but supposedly success- 

 ful "takes" having been obtained, showed redness and slight 

 swelling at the points of inoculation with pulp from No. 23, but 

 nothing at all characteristic or strongly suggestive of smallpox 

 or vaccinia. 



Monkey 16. — This monkey had been unsuccessfully inoculated 

 in November with vaccine triturated in 1 per cent phenol in 0.85 

 per cent saline solution and so kept for two weeks, no "take" 

 resulting. Inoculated with "pulp" from the lesions of monkey 

 23 on January 7, the animal had a rise of temperature beginning 

 the sixth day thereafter as shown by chart 7. 



Beginning on the seventh day after inoculation, the animal 

 showed marked induration and some oedema of and about the 

 sites of inoculation and thick dry scabs formed. The induration 

 was deep. On January 16 (tenth day after inoculation) the scabs 

 were lifted, the areas beneath curetted, and the pulp so obtained 

 used to inoculate monkeys 25, 19, 28, and 3, the first three of 

 which have been discussed, and the last being a monkey vacci- 

 nated in October. None of them showed general or local 

 disturbance. 



