440 



The Philippine Journal of Science 



1913 



This experiment indicates that the virus in vesicle contents, 

 although attenuated by storage and further attenuated by pas- 

 sage, was still recognizably active in this animal, but not suffi- 

 ciently so to survive another passage. 



SUMMARY 



1. Fresh vesicle contents from a case of human variola is 

 capable, when inoculated into abrasions or scarifications on non- 

 vaccinated monkeys, of producing variola inoculata in those mon- 

 keys, the disease being marked by fever and by primary and 

 secondary lesions. 



2. Such vesicle contents kept at ice-chest temperature for 

 twenty-three days loses most of its virulence, but may still, in 



Chart 7. — Temperature chart of monkey 16. 



a proportion of instances, produce a mild and atypical variola 

 inoculata, which in turn and in further modified form may be 

 passed to other monkeys. 



3. Active and fresh vesicle contents inoculated on vaccinated 

 monkeys may produce a fever closely resembling that of variola 

 inoculata in the monkey and a condition permitting of inter- 

 pretation as variola sine exanthemate in the monkey. 



4. Smallpox scabs or disks from man or monkey possess but 

 a low degree of virulence, or very quickly lose their virulence. 



5. When inoculation of such scabs does result in the produc- 

 tion of infection this may be manifested only locally at the site 

 of inoculation (case 24) . In other words, the "B" part of small- 

 pox virus survives longest in scabs. 



