viii, B, c Barber: Examination of Artesian Wells 



447 



The results of these tests are given in detail in Tables IV to 

 XVI, inclusive. Samples were for the most part placed in the 

 media at the well, but some samples were taken to the laboratory 

 for further examination, as was done in the case of the flowing 

 wells. Enough water was pumped out to flush out the pipe 

 before samples were taken, although the wells were in contin- 

 uous use during the time of day when samples were collected. 

 Two wells which flowed intermittently are included in this list. 



In Tables IV to XVI the fractions under the heading "gas" 

 refer to the proportion of gas formed in the closed arm of the 

 fermentation tube. 



Table IV. — Alabang Agricultural Experiment Station, Rizal Province, 



Well 1. 



Water, fresh ; depth of wells, 167 meters ; in use since July, 1908. Water of several wells 

 pumped to collecting tank. Samples (taken April 11, 1913) from pipe leading from collecting 



tank. 



Nutrient medium. 



Glucose-broth fermentation 

 tube 



do ..,.. _ 



Glucose agar 



do 



Plain agar 



do 



do 



Litmus-lactose fermentation 

 tube 



do 



Litmus-lactose agar 



do 



Bile-lactose fermentation 

 tube 



do 



Litmus-lactose agar plate 



Result. 



24 hours at 36°. 



n to 

 II 



48 hours at 

 from 86-37°. 



01 V 



.2 <= 



.2 3 



3 days at 

 from 36-87°. 



.2 3 



0.2 





6 days. 



* + about 3. 



* -f- 5 or more. 



■^ Many colonies present. 



■' -r about 4. 

 ' 4- about 6. 

 ' + about 7. 



.2 3 

 o o 



(b) 



+ 



+5 

 (0 



+ 



+ 

 -I- 

 -I- 

 + 







+ 



