450 



The Philippine Journal of Science 



191S 



This water is in all probability polluted. From the 0.1 cc. sample in the 

 glucose-broth fermentation tube a bacillus was isolated with coli-like mor- 

 phology, motility doubtful. It produced acid and gas in lactose-, dulcite-, 

 saccharose-, and mannite-litmus agars, and gas in bile-lactose agar. Nine- 

 tenths of the closed arm of a glucose-broth fermentation tube was filled with 

 gas, about half of which was absorbed by NaQH. See Table XVII. 



Table VIIL— Water from ivells at Fort William McKinley, Rizal Province." 



Samples taken April 9, 1913 ; water, sweet ; temperature, 30» C. ; near Paaig River ; depth 

 from 225 to 274 meters ; water collected from several wells, about 2,270,000 liters used daily • 

 samples were taken from the main leading from the collecting tank to the post. 



1 

 c 



i 



Nutrient medium. 



Result. 



1 



24 hours at 36° 



. 48 hours at 36° 



. 3 days at 36°. 



1 



8 days. 1 



1 



J 



■5 

 <! 



II 



J 



1 



1 

 1 



*5 



< 



(d) 

 (?) 

 (-) 

 (1) 



u . 



O in 



ss 

 If 



+ 

 + 



-1- 

 -1- 

 + 



a, 



+ 



-r 



+ 

 1 



+ 



1 



+ 

 + 

 



+ 



A 



(») 

 («) 



A 



3' 



< 



u . 



If 



1 



i 



li 



So 

 If 



cc. 

 0.1 



1 

 5 

 1 



Glucose-broth fermentation 

 tube. 



Ta 



+ 

 + 

 

 



+ 









 



(=) 



+ 

 (?) 







+ 

 + 

 + 



+ 



-r 



+ 

 + 

 + 

 -h 



+ 

 + 

 + 

 (0 



/» 



+ 

 + 

 

 



TO 



+ 



(=) 

 (e) 



A 



+ 

 (") 

 (d) 

 (d) 



4- 



+ 

 + 

 + 

 + 



(b) 



+ 



+ 



+ 

 -t- 



+ 



+ 



A 

 \% 

 A 

 + 

 + 

 



+ 



A 

 A 

 A 



+ 



A 

 A 

 A 



+ 



(d) 

 (d) 

 (d) 



i "T 

 + 



+ 

 + 



+ 



+ 



-r 

 + 



+ 

 + 

 + 



do 



do 



Glucose agar 



6 



0.1 



do 



Plain agar -. 



1 

 0.1 



do. 



Litmus-lactose fermenta- 

 tion tube 



1 



5 



do 



do 



5 

 0.1 



Litmus-lactose agar 



Bile-lactose fermentation 

 tube 



1 



do 



5 



do 



1 



Litmus-lactose agar plate.. 



1 



Plain agar plate 







(.) 























. 



J 



' 















mzL 





» See Table XVII. 

 * -f about 10. 

 " + about 100. 

 <* Alkaline. 



• SUght. 



' 30+ (overgrown) . 



« 46+ (overgrown) . 



