VIII, B, 6 Barber: Examination of Artesian Wells 



455 



Table XVI. — Water from well in Taytay, Rizal Province^ 



Samples taken April 9, 1913 ; water, slightly brackish ; temperature, 29°.6 C. ; standing water 

 only from overflow ; depth, 156 meters ; in use since June, 1911. 



Amount of water sown. 



Nutrient medium. 













Result. 













24 hours at 36°. 



48 hours at 36°. 



3 days at 36°. 



8 days at 36°. 



n 

 a 



3 



< 



OS 



11 



a 



3 

 'S 



< 



II 



O 



73 

 < 



OS 



IS 

 §1 



61 



CO 



a 



•a 

 o 

 < 



sg 

 II 

 61 



ee. 





























0.1 



Glucose-broth fermentation 

























i 



1 



tube 









-t- 

 -t- 









+ 









+ 

 + 









+ 

 + 



do 



5 



do 



\% 





-f 



\i 





+ 



f8 





^- 



I's 





+ 



1 

 6 





+ 

 4 





+ 

 + 



+ 



4- 







+ 



+ 

 + 





-j- 



+ 

 + 





+ 



do 



1 

 6 







 





+ 

 -f 





 





+ 





 





^ 





 





+ 

 + 



do 



0.1 



Lactose-broth fermentation 



























1 



e 



1 



tube - _ - . 





 

 



+ 







+ 

 + 



+ 





 



+ 







+ 

 + 

 + 





 



+ 



(b) 



+ 

 + 





 



(•>) 



+ 

 (") 



+ 



do 



do 



Lactose-bile fermentation 





tube 



A 





+ 



A 





+ 



A 





+ 



^% 





+ 



6 



do - 









+ 









+ 



(0 





+ 



ft 





+ 



5 



Litmus-lactose agar 



+ 



+ 



+ 



-1- 



+ 



+ 



+ 



+ 



4- 



+ 



(•>) 



+ 



1 









d45 





















1 



Plain agar plate 







254 













































' See Table XVII. 



*• Alkaline. 



<^ Slight. 



■•M Acid. 



Further tests were made of a number of samples which showed 

 gas and acid in litmus-lactose agar or in bile media, in order to 

 determine if Bacterium coli was present. Such samples from 6 

 different wells were transferred to litmus-lactose agar. No acid 

 or gas appeared in stab cultures, although transfers were made 

 directly from the original bile or litmus-lactose samples which 

 had shown gas or gas and acid. In 3 wells colonies were isolated 

 which showed gas and acid in litmus-lactose agar. These colo- 

 nies were tested on various media, and the results are given in 

 Table XVII. 



