480 ^^^6 Philippine Journal of Science ms 



Underbill's work, however, does not seem to have been considered 

 by subsequent writers. Popielski ^* and his associates ascribe 

 the active physiological principle of intestinal extracts and of . 

 Witte "peptone" to an alcohol-soluble substance "vasodilatine." 

 In a recent paper, Popielski says:^" 



Vasodilatin ist ein chemisch einheitliches Korper; es ist weder Cholin, 

 noch /3-Imidazolathylamin, noch entsteht es durch Zerfall von Cholin. 



The experiments, to be reported here, were carried out several 

 years ago because of the suggestion by Krehl of the possibility 

 of a contaminating substance being responsible for the pyrexial 

 effects ascribed to the proteoses. The association of proteoses 

 and of fever with the anaphylactic reaction has made them 

 timely, and I have thought it worth while to present them in 

 the present paper. The original problem was simply an inquiry 

 as to whether pyrexial effects result from the injection of pro- 

 teoses, prepared by gentle hydration from pure proteins without 

 subsequent drastic treatment. Now, however, the results must 

 be considered also in their relation to the subject of anaphylaxis, 



TECHNIQUE OF THE EXPERIMENTS 



A description of the proteoses used in the present experiments 

 has been given in an earlier paper *" on the pharmacological 

 action of these products on the heart. The proteoses were made 

 from four-times reprecipitated caseinogen, from recrystallized 

 edestin, and from thoroughly washed pig fibrin, by digestion 

 with a very active scale pepsin prepared by me. The proteoses 

 were salted out with ammonium sulphate, redissolved, and again 

 salted out. The salt was removed by dialysis for two weeks, 

 with thymol as the preservative. The dialyzate filtrate was con- 

 centrated in vacuo as 50° to a sirup, precipitated with 95 per 

 cent alcohol, and dehydrated with hot absolute alcohol. The 

 mixed proteoses were obtained as a fine white powder which 

 was easily soluble in water. The filtrates from the alcohol-pre- 

 cipitated proteoses were concentrated, precipitated with hot 

 absolute alcohol, and, on drying, yielded a fine white powder, 

 evidently proteose. 



These alcohol-soluble proteoses may be prepared from Witte 

 peptone or other mixed proteose preparations by extracting with 



''Arch. f. d. ges. Physiol. (Pfliiger) (1907), 120, 451; ibid. (1908), 121, 

 239; ibid. (1909), 126, 483; also Czubalski, ibid. (1908), 121, 395; Gizelt, 

 ibid. (1908), 123, 540. 



" Zeitschr. f. Immunitdtsforsch., Orig. (1913), 18, 562. 



'"Gibson and Shultz, Journ. Pharm. & Exp. Therap. (1909-10) 1. 



