VIII, B, 6 Boynton: Rinderpest Blood in Water Leech 511 



animal showed a rise in temperature on the morning of July 19, 

 diarrhoea with inappetence on July 22, and died during the fore- 

 noon of July 24. The symptoms and lesions gave conclusive 

 evidence that it had contracted rinderpest and died of that 

 disease. 



On July 16, 1912, at 11.00 a. m., several leeches were allowed 

 to feed on bull 3402 in the second day of temperature of an 

 attack of rinderpest. As soon as the leeches had become en- 

 gorged, they were placed in water and kept in a cool place. The 

 infectiveness of the blood contained in these leeches was deter- 

 mined at various intervals by testing upon cattle as shown in 

 experiments 3 to 6. 



Experiment 3. — On July 17, 1912, at 11.00 a. m., 2 leeches 

 which had fed on July 16 were placed in 100 cubic centimeters 

 of physiological salt solution which caused them to disgorge the 

 blood which they had held for twenty-four hours. This mixture 

 of blood and salt solution was injected subcutaneously into bull 

 3405. The animal had a rise in temperature on July 22, de- 

 veloped diarrhoea and inappetence on July 26, and died on July 27. 

 From the symptoms and lesions it was concluded that the animal 

 had contracted rinderpest and died of that disease. 



Experiment U. — On July 21, 1912, several of the leeches which 

 had fed on July 16 died and disgorged blood into the water in 

 which they were being kept. The mixture of water and blood 

 was given as a drench to bull 3404. This animal showed a febrile 

 temperature on July 31, which was ten days after receiving the 

 drench. Diarrhoea with inappetence appeared on August 6 and 

 continued until August 10, after which the animal gradually 

 recovered. The case presented all the symptoms of a severe 

 attack of rinderpest. At a later date the animal received virulent 

 blood and was proved to be immune, thus showing that it had 

 passed through the disease. 



Experiment 5. — On July 21, 1912, the dead leeches which were 

 mentioned in experiment 4, and which had fed on an infected 

 animal on July 16, were thoroughly disintegrated in a mortar 

 containing physiological salt solution, and the fluid was injected 

 subcutaneously into bull 3400. This animal showed a high tem- 

 perature on the evening of July 27, diarrhoea with inappetence 

 on July 31, and died during the forenoon of August 7. From 

 the symptoms and lesions it was concluded that this animal had 

 contracted rinderpest and had died of that disease. 



Experiment 6. — On August 2, 1912, two leeches which had fed 

 on July 16, seventeen days previously, were placed in physiolog- 



