VIII. B. s Boynton: Rinderpest Blood in Water Leech 513 



Experiment 10. — On August 16, 1912, the water in which the 

 leeches of experiment 9 had been kept since July 29 — a period 

 of twelve days — was given as a drench to bull 3488. This animal 

 suffered no ill effects from the drench. It was later infected 

 with rinderpest and died, showing that it had been susceptible 

 to the disease at the time it had received the drench. 



On August 17, 1912, twenty leeches were allowed to feed upon 

 bull 3492 during the third day of temperature of an attack of 

 rinderpest, and were placed in water in three different con- 

 tainers and kept in a cool place. The infectiveness of the blood 

 contained in these leeches was tested on cattle at various periods 

 in experiments 11 to 14, 



Experiment 11. — On August 24, 1912, two leeches which had 

 fed on August 17, seven days previously, were placed in 50 cubic 

 centimeters of physiological salt solution, which caused them 

 to disgorge. The mixture of blood and salt solution was in- 

 jected into animal 3494, This animal showed a rise in temper- 

 ature on September 3, ten days after receiving the injection. 

 Diarrhoea developed on September 4, and inappetence on Sep- 

 tember 5. The animal showed a subnormal temperature of 36°, 2 

 C. on the afternoon of September 6, and died that evening. 

 Autopsy showed typical lesions of rinderpest. It was concluded 

 that this animal had contracted a fatal attack of rinderpest. 



Experiment 12. — On August 24, 1912, the water in which 8 

 leeches had been kept since August 17, an interval of seven days, 

 was given by drench to bull 3495. The animal suffered no ill 

 effects from the material. This animal was used in a subsequent 

 experiment in which it was shown to be susceptible. 



Experiment 13. — On August 27, 1912, three leeches which had 

 fed on August 17, ten days previously, were placed in 75 cubic 

 centimeters of physiological salt solution, which caused them to 

 disgorge. The mixture of blood and salt solution was injected 

 into bull 3488, This animal suffered no ill effects from the 

 injection, and at a later date was proved to be susceptible to 

 rinderpest. 



Experiment llf.—0r\ August 27, 1912, the water in which 

 several leeches had been kept since August 17 — a period of ten 

 days — was given by drench to bull 3491, This animal suffered 

 no ill effects from the material, but at a later date was proved 

 to be susceptible to rinderpest. 



On November 8, 1912, five leeches were allowed to feed on 

 cow 3516, in the second day of febrile temperature of an attack 

 of rinderpest, and were placed in water in a cool place. The 



