514 The Philippine Journal of Science mis 



ineffectiveness of the rinderpest virus in the blood contained in 

 these leeches w^as tested upon cattle in experiments 15 to 17. 



Experiment 15. — On November 18, 1912, one leech, which had 

 fed on November 8, ten days previously, was placed in 50 cubic 

 centimeters of physiological salt solution which caused it to dis- 

 gorge. The mixture of blood and salt solution was injected 

 subcutaneously into bull 3514. This animal showed an initial 

 rise of temperature on November 23, developed diarrhoea on 

 November 24, and inappetence on November 26. It displayed 

 a subnormal temperature of 36°. 7 C. on the morning of No- 

 vember 29, and died during the day. Autopsy shov/ed typical 

 lesions of rinderpest. It was thus proved positively that this 

 animal had suffered a fatal attack of rinderpest. 



Experiment 16. — On November 20, 1912, one leech, which 

 had fed on November 8, twelve days previously on an infected 

 animal, was placed in 50 cubic centimeters of physiological salt 

 solution, which caused it to disgorge. The mixture of blood 

 and salt solution was injected subcutaneously into bull 3518. 

 This animal showed the initial rise of temperature on Novem- 

 ber 26, which continued for several days, gradually subsiding 

 to normal. The animal showed no diarrhoea nor inappetence. 

 Blood was drawn from it and injected into a susceptible bull 

 which developed a severe case of rinderpest and died. This 

 proved that bull 3518 had been infected with a mild type Of 

 rinderpest, but had been able to transfer a severe type of the 

 disease to another animal. 



Experiment 17. — On November 23, 1912, one leech, which 

 had fed on November 8, fifteen days previously, was placed in 

 50 cubic centimeters of physiological salt solution, which caused 

 it to disgorge. The mixture of blood and salt solution was 

 injected subcutaneously into bull 3524. This animal showed an 

 initial rise of temperature on November 28, developed diarrhoea 

 with partial inappetence on December 2, and died during the 

 daytime of December 6. The autopsy revealed marked lesions 

 of rinderpest. Therefore, this animal had a fatal attack of 

 rinderpest. 



On November 30, 1912, four leeches were allowed to feed on 

 cow 3524, in the second day of febrile temperature of an attack 

 of rinderpest, and were placed in water in a cool place. The 

 infectiveness of the blood retained in these leeches was tested 

 upon cattle at various intervals as shown in experiments 18 

 and 19. 



Experiment 18. — On December 10, 1912, one leech which had 

 fed November 30, ten days previously, was placed on some green 



