516 '^^^ Philippine Journal of Science isis 



concerning which there had been considerable doubt, owing to 

 the absence of well-marked cases. 



Experiment 21. — On February 24, 1913, two leeches which 

 had fed on the sick carabao at Magarao, Ambos Camarines, on 

 January 15, forty days previously, were disintegrated in a mortar 

 and the blood was injected into bull 3548. The animal appar- 

 ently suffered no ill effect from the injection but was proved 

 susceptible to rinderpest at a later date. 



On January 22, 1913, twelve leeches were allowed to feed on 

 cow 3535, on the second day of febrile temperature of an attack 

 of rinderpest, and then placed in water in a cool place. The 

 blood contained in these leeches was tested upon cattle in ex- 

 periments 22 to 27. 



Experiment 22. — On February 11, 1913, two leeches which 

 had fed on January 22, twenty days previously, were placed in 

 100 cubic centimeters of physiological salt solution, which caused 

 them to disgorge blood. This mixture of blood and salt solution 

 was injected into bull 3564. The animal suffered apparently no 

 ill effects from the injection. At a later date it was proved to 

 be susceptible to rinderpest. 



Experiment 23. — On February 16, 1913, two leeches which 

 had fed on January 22, twenty-five days previously, were placed 

 in 75 cubic centimeters of physiological salt solution, which 

 caused them to disgorge. This mixture was injected subcu- 

 taneously into bull 3566. The animal showed an initial rise of 

 temperature on February 22, developed inappetence on February 

 27, and diarrhoea on March 1. The inappetence continued to 

 March 4, when the animal again began to eat, but the diarrhoea 

 continued until March 7. This animal gradually recovered. 

 From the symptoms it was concluded that this animal experienced 

 an attack of rinderpest. 



Experiment 2U. — On February 22, 1913, one leech, which had 

 fed on January 22, thirty-one days previously, was caused to 

 disgorge by means of salt solution. The mixture of blood and 

 salt solution was injected into bull 3549. This injection had 

 apparently no ill effect upon the animal. Its susceptibility to 

 rinderpest was proved later. 



Experiment 25. — On February 26, 1913, five leeches, which 

 had fed on cow 3535 on January 22, thirty-five days previously, 

 were allowed to feed on bull 3568. This animal suffered no ill 

 effects from the feeding. It was proved susceptible to rinderpest 

 at a later date. 



Experiment 26. — On March 3, 1913, four leeches which had 



